EZH1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56358

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (93%), Monkey (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A portion of amino acids 350-400 of human EZH1 was used as the immunogen.

Reactivity Notes

Immunogen sequence similarity with rat and dog (86%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EZH1 Antibody - BSA Free

Western Blot: EZH1 Antibody [NB100-56358]

Western Blot: EZH1 Antibody [NB100-56358]

Western Blot: EZH1 Antibody [NB100-56358] - WB detection of EZH1 protein in (A ) Ramos cells lysate, (B) Daudi cells lysate and (C) Peptide blocked - Daudi cells lysate by using EZH1 antibody at a concentration of 2 ug/mL.
Western Blot: EZH1 Antibody [NB100-56358]

Western Blot: EZH1 Antibody [NB100-56358]

Western Blot: EZH1 Antibody [NB100-56358] - Detection of EZH1 in mouse (lane 1) and human (lane 2) spleen lysate using this antibody at 2 ug/mL.
Western Blot: EZH1 Antibody [NB100-56358]

Western Blot: EZH1 Antibody [NB100-56358]

Western Blot: EZH1 Antibody [NB100-56358] - 15 ug of HeLa nuclear extract on PVDF membrane. Image from verified customer.

Applications for EZH1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

2 - 4 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 3 using NB100-56358 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EZH1

EZH1 is a human homolog of the Drosophila gene Enhancer of zeste E(z), a member of the polycomb group of transcriptional repressors. In mice, EZH1 is a regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin. The strong sequence conservation between species suggests potential role for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that stably maintain heterochromatin.

Long Name

Histone-lysine N-methyltransferase EZH1

Alternate Names

EC 2.1.1.356, ENX-2, KIAA0388

Entrez Gene IDs

2145 (Human); 14055 (Mouse)

Gene Symbol

EZH1

UniProt

Additional EZH1 Products

Product Documents for EZH1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EZH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EZH1 Antibody - BSA Free

Customer Reviews for EZH1 Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa nuclear extract on PVDF membrane
    Species: Human
    Verified Customer | Posted 01/31/2014
    EZH1 Antibody - BSA Free NB100-56358

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