F-Spondin/SPON1 Antibody - BSA Free

Novus Biologicals | Catalog # NB300-339

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ICC/IF (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Chicken

Format

BSA Free
View all F-Spondin/SPON1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide, corresponding to amino acids 549-807 of SPON1.

Reactivity Notes

Cross-reacts with Human, Mouse, Rat.

Localization

Secreted, Extracellular Matrix

Specificity

SPON1

Clonality

Polyclonal

Host

Chicken

Scientific Data Images for F-Spondin/SPON1 Antibody - BSA Free

Western Blot: F-Spondin/SPON1 Antibody [NB300-339]

Western Blot: F-Spondin/SPON1 Antibody [NB300-339]

Western Blot: F-Spondin/SPON1 Antibody [NB300-339] - E coli-derived fusion protein as test antigen. Affi-anti- SPON1 IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-SPON1 )

Applications for F-Spondin/SPON1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:200 - 1:500
Application Notes
In Western Blot predicted molecular weight 100.9 kDa. Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.91 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: F-Spondin/SPON1

SPON1 is a member of a subgroup of the thrombospondin type 1 (TSR) class molecules, defined by two domains of homology, the FS1/FS2 and TSR domains. The TSRs of SPON1 proteins are typical of class 2 TSRs. SPON1, which is similar to thrombospondin, is a extracellular matrix attached molecule that promotes neurite outgrowth and inhibits angiogenesis. Analysis of gain and loss of function experiments reveal that SPON1 is required for accurate pathfinding of embryonic axons, and plays a dual role in patterning axonal trajectories. It promotes the outgrowth of commissural and inhibits the outgrowth of motor axons, and has also been implicated in inflammatory processes in the nervous system.

Alternate Names

FSpondin, SPON1, VSGP

Entrez Gene IDs

104062 (Mouse); 64456 (Rat)

Gene Symbol

SPON1

Additional F-Spondin/SPON1 Products

Product Documents for F-Spondin/SPON1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for F-Spondin/SPON1 Antibody - BSA Free

Chicken products cannot be exported to Canada.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

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