Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 034 expressed in HEK293

Format

Azide and BSA Free
View all Coagulation Factor IX Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Factor IX (Accession#: AAB59620.1; Met 1-Thr 461).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Factor IX Antibody (034) - Azide and BSA Free

Western Blot: Factor IX Antibody (034) [NBP2-89906]

Western Blot: Factor IX Antibody (034) [NBP2-89906]

Western Blot: Factor IX Antibody (034) [NBP2-89906] - Lane A: A431 Whole Cell Lysate Lane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:52 kDa Observed band size:55 kDa
Immunoprecipitation: Factor IX Antibody (034) [NBP2-89906]

Immunoprecipitation: Factor IX Antibody (034) [NBP2-89906]

Immunoprecipitation: Factor IX Antibody (034) [NBP2-89906] - Lane A:0.5 mg A431 Whole Cell Lysate Lane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-F9 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-F9 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the ECL technique. Performed under reducing conditions.Predicted band size: 55 kDa Observed band size :55 kDa

Applications for Factor IX Antibody (034) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

0.1-0.5 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coagulation Factor IX

Factor IX encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Alternate Names

Christmas Factor, F9, FIX, HEMB, P19, Plasma Thromboplastic Component, PTC, THPH8

Gene Symbol

F9

Additional Coagulation Factor IX Products

Product Documents for Factor IX Antibody (034) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Factor IX Antibody (034) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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