FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13675

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL127 expressed in HEK293

Format

Azide and BSA Free
View all FAK Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr576 of human FAK. The exact sequence is proprietary.

Modification

p Tyr576

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
FAK [p Tyr576] Antibody (HL127)

Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -

Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK antibody [HL127] (NBP3-13675) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
FAK [p Tyr576] Antibody (HL127)

Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -

Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

Western Blot: FAK [p Tyr576] Antibody (HL127) [NBP3-13675] -

Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FAK (phospho Tyr576) antibody [HL127] (NBP3-13675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAK

FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Gene Symbol

PTK2

Additional FAK Products

Product Documents for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FAK [p Tyr576] Antibody (HL127) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail