FAK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84750

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine

Cited:

Porcine

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRE

Reactivity Notes

Porcine reactivity reported in the scientific literature (PMID: 30257480).

Marker

Focal Adhesion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FAK Antibody - BSA Free (NBP1-84750) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-FAK Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FAK Antibody - BSA Free

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]

Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750]

Immunocytochemistry/Immunofluorescence: FAK Antibody [NBP1-84750] - Staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites. Antibody staining shown in green.
Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human testis shows moderate to cytoplasmic positivity in spermatogonia and Leydig cells.
Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750]

Immunohistochemistry-Paraffin: FAK Antibody [NBP1-84750] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
FAK Antibody

Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750] -

Immunocytochemistry/ Immunofluorescence: FAK Antibody [NBP1-84750] - Membrane surface receptor expression & metabolomic analysis of cells grown on porcine breast fatty tissue ECM hydrogel. Integrin beta 1 (a) or beta 4 (b) receptor (green) & FAK (red) expression in MM231 cells cultured on the ECM hydrogel-coated coverslips were assessed by IF. Phalloidin staining (white) of F-actin was used to contour the cells. Scale bars, 10 μm. (c) MS imaging of cross sections of the medium-conditioned blank (upper panels) & MM231 cell culture-laden (bottom panels) hydrogel scaffolds. (d) Examples of ROC (upper) & box-and-whiskers (bottom) plots from the MS imaging analysis, showing data for a compound with m/z 663.024, where differences between the hydrogel samples cultured with or without MM231 cells are clearly evident. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30257480), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for FAK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FAK

FAK (Focal Adhesion Kinase) is a non-receptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness.

Long Name

Focal adhesion kinase 1

Alternate Names

FADK1, PTK2

Gene Symbol

PTK2

Additional FAK Products

Product Documents for FAK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FAK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FAK Antibody - BSA Free

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail