Family with Sequence Similarity 105, Member B (FAM105B), also known as OTULIN, is a 352 amino acid (aa) member of the ovarian tumor protein superfamily with a predicted molecular weight of 40 kDa. The mouse FAM105B/OTULIN ortholog shares 89% aa sequence identity with the human protein. It is a C65 type peptidase that has high specificity for methionine-1-linked linear Ubiquitin chains and contributes to the regulation of NFkB signaling.
FAM105B/OTULIN Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-29109
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
between 75 and 125
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FAM105B/OTULIN Antibody - BSA Free
Immunoprecipitation:FAM105B/OTULIN AntibodyNBP3-29109] -
Immunoprecipitation:FAM105B/OTULIN AntibodyNBP3-29109] -Detection of human OTULIN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-OTULIN antibody (NBP3-29109 lot 1) used for IP at 2 µg per reaction. OTULIN was also immunoprecipitated by rabbit anti-OTULIN.. For blotting immunoprecipitated OTULIN, NBP3-29109 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.Applications for FAM105B/OTULIN Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate pH 7 -8.
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: FAM105B/OTULIN
Long Name
Family with Sequence Similarity 105, Member B
Alternate Names
OTULIN
Gene Symbol
OTULIN
Additional FAM105B/OTULIN Products
Product Documents for FAM105B/OTULIN Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FAM105B/OTULIN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
