FANCD2 Antibody (1290D) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80729

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Simple Western, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1290D expressed in HEK293

Format

Azide and BSA Free
View all FANCD2 Primary Antibodies »

Product Specifications

Immunogen

This FANCD2 Antibody (1290D) - Azide and BSA Free was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FANCD2 Antibody (1290D) - Azide and BSA Free

Western Blot: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]

Western Blot: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]

Western Blot: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729] - Total protein from HeLa and K562 cells (Molecular weight: 164.1 KDa) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.1 ug/ml FANCD2 Antibody (1290D) (catalog number NBP2-54808) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunohistochemistry-Paraffin: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]

Immunohistochemistry-Paraffin: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]

Immunohistochemistry-Paraffin: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Antibody (1290D) (Catalog # NBP2-54808) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM)
Simple Western: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]

Simple Western: FANCD2 Antibody (1290D)Azide and BSA Free [NBP2-80729]

Simple Western: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729] - Detection of Human FANCD2 by Simple Western using FANCD2 Antibody (1290D) in HeLa and K562 cell lysate. Image from the standard format of this antibody.
Western Blot: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]

Western Blot: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729]

Western Blot: FANCD2 Antibody (1290D) - Azide and BSA Free [NBP2-80729] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions. Image from the standard format of this antibody.

Applications for FANCD2 Antibody (1290D) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Simple Western

1 ug/ml

Western Blot

0.1 - 0.5 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FANCD2

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K.,... Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B.,... Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Long Name

Fanconi Anemia, Complementation Group D2

Alternate Names

FA4, FACD, FAD, FAD2, FANCD

Gene Symbol

FANCD2

Additional FANCD2 Products

Product Documents for FANCD2 Antibody (1290D) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for FANCD2 Antibody (1290D) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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