FANCD2 Antibody (1290D) - BSA Free

Novus Biologicals | Catalog # NBP2-54808

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1290D expressed in HEK293

Format

BSA Free
View all FANCD2 Primary Antibodies »

Product Specifications

Immunogen

This FANCD2 Antibody (1290D) was developed against human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

164.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FANCD2 Antibody (1290D) - BSA Free

Knockout Validated: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]

Western Blot: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]

Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and FANCD2 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.1 ug/mL of Rabbit Monoclonal (Catalog # NBP2-54808) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FANCD2 at approximately 150 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Simple Western: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]

Simple Western: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]

Simple Western: FANCD2 Antibody (1290D) [NBP2-54808] - Detection of Human FANCD2 by Simple Western using FANCD2 Antibody (1290D) in HeLa and K562 cell lysate.
Immunohistochemistry: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]

Immunohistochemistry: FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]

Immunohistochemistry: FANCD2 Antibody (1290D) [NBP2-54808] - FANCD2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human FANCD2 Antibody (1290D) (Catalog # NBP2-54808) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte(TM) HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.
Flow (Intracellular): FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]

Flow (Intracellular): FANCD2 Antibody (1290D) - BSA Free [NBP2-54808]

Flow (Intracellular): FANCD2 Antibody (1290D) [NBP2-54808] - An intracellular stain was performed on HeLa Cells with FANCD2 Antibody (1290D) (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).
Western Blot: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]

Western Blot: FANCD2 Antibody (1290D)BSA Free [NBP2-54808]

Western Blot: FANCD2 Antibody (1290D) [NBP2-54808] - Total protein from HeLa and K562 cells (Molecular weight: 164.1 KDa) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.1 ug/ml FANCD2 Antibody (1290D) (catalog number NBP2-54808) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Applications for FANCD2 Antibody (1290D) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug/ml

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

Simple Western

1 ug/ml

Western Blot

0.1 - 0.5 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP2-54808 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FANCD2

Fanconi Anemia (FA) is an autosomal-recessive cancer-prone disorder characterized by congenital defects, progressive bone marrow failure, increased chromosomal breakage, defective DNA repair and cellular hypersensitivity to mitomycin C. Fanconi Anemia Complementation Group D (FANCD) is comprised of two separate proteins, FANCD1/BRCA2 and FANCD2. LYRIC/MTDH, an RNA binding protein, regulates expression of FANCD2 and FANCD1 (1). FANCD2 is mapped to chromosome 3p25.3, has a theoretical molecular weight of 166 kDa and is localized in the nucleus. FANCD2 is involved in the regulation of DNA-binding transcription factor activity and DNA stability through accurate and efficient pairing of homologs during meiosis to promote repair of double-strand DNA breaks (2). Ubiquitination is required for FANCD2 to bind to chromatin. In response to DNA damage, FANCD2 is monoubiquinated to result in colocalization with other proteins (BRCA1 and BRCA2) involved in homology-directed DNA repair in the nucleus and is deubiquitinated upon DNA repair completion.

References

1. Bi, J., Areecheewakul, S., Li, Y., Yang, S., Zhang, Y., Ebeid, K.,... Meng, X. (2019). MTDH/AEG-1 downregulation using pristimerin-loaded nanoparticles inhibits Fanconi anemia proteins and increases sensitivity to platinum-based chemotherapy. Gynecol Oncol, 155(2), 349-358. doi:10.1016/j.ygyno.2019.08.014

2. Balcerek, J., Jiang, J., Li, Y., Jiang, Q., Holdreith, N., Singh, B.,... Tong, W. (2018). Lnk/Sh2b3 deficiency restores hematopoietic stem cell function and genome integrity in Fancd2 deficient Fanconi anemia. Nat Commun, 9(1), 3915. doi:10.1038/s41467-018-06380-1

Long Name

Fanconi Anemia, Complementation Group D2

Alternate Names

FA4, FACD, FAD, FAD2, FANCD

Gene Symbol

FANCD2

Additional FANCD2 Products

Product Documents for FANCD2 Antibody (1290D) - BSA Free

Certificate of Analysis

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Product Specific Notices for FANCD2 Antibody (1290D) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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  • FANCD2 Antibody (1290D)
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Human
    Species: Human
    Verified Customer | Posted 09/08/2017
    D2 Foci PD20 ce;;s
    FANCD2 Antibody (1290D) - BSA Free NBP2-54808

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