Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 4484 to 4534 of human FAT Tumor Suppressor Homolog 1 using the numbering given in entry NP_005236.2 (GeneID 2195).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FAT1 Antibody - BSA Free

Western Blot: FAT1 Antibody [NBP2-32275]

Western Blot: FAT1 Antibody [NBP2-32275]

Western Blot: FAT1 Antibody [NBP2-32275] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-FAT1 antibody NBP2-32275 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for FAT1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2000 - 1:10000

Reviewed Applications

Read 1 review rated 4 using NBP2-32275 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FAT1

The Drosophila FAT 1 gene does not belong to the classical cadherin gene family yet encodes a transmembrane protein containing 34 cadherin repeats in association with a number of other motifs. The Drosophila FAT locus encodes a tumor suppressor gene and recessive (loss-of-function) mutations lead to hyperplastic overgrowth of the imaginal discs, indicating that contact dependent cell interactions may play an important role in regulating growth and cell adhesion. This excessive cell proliferation occurs while maintaining normal epithelial organization and differentiation potential. Expressed in many epithelial and some endothelial and smooth muscle cells. Humans contain many homologs of FAT including FAT1, or hFAT

Alternate Names

FAT tumor suppressor 1, FAT tumor suppressor homolog 1 (Drosophila)

Gene Symbol

FAT1

UniProt

NP_005236.2 (Human)

Additional FAT1 Products

Product Documents for FAT1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FAT1 Antibody - BSA Free

Customer Reviews for FAT1 Antibody - BSA Free (1)

4 out of 5
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  • FAT1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: H1944 lung adenocarcinoma cell line
    Species: Human
    Verified Customer | Posted 11/26/2018
    FAT1 Antibody - BSA Free NBP2-32275

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