FBXO3 Antibody (PSH04-09)

Novus Biologicals | Catalog # NBP3-32334

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH04-09 expressed in HEK293
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Product Specifications

Immunogen

Recombinant protein within human FBXO3 aa 1-450 / 471. (Uniprot: Q9UK99)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FBXO3 Antibody (PSH04-09)

FBXO3 Antibody (PSH04-09) Western Blot: FBXO3 Antibody (PSH04-09) [NBP3-32334]

Western Blot: FBXO3 Antibody (PSH04-09) [NBP3-32334]

Western blot analysis on different lysates with NBP3-32334 at 1/2,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MCF7 cell lysate Lane 5: SK-Br-3 cell lysate Lane 6: JAR cell lysate Lane 7: HepG2 cell lysate Lane 8: NIH/3T3 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 55 kDa Observed band size: 55/48 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32334 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for FBXO3 Antibody (PSH04-09)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FBXO3

Alternate Names

DKFZp564B092, F-box protein 3, F-box protein FBX3, FBX3FBAF-box only protein 3

Gene Symbol

FBXO3

Additional FBXO3 Products

Product Documents for FBXO3 Antibody (PSH04-09)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FBXO3 Antibody (PSH04-09)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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