Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 549]

Novus Biologicals | Catalog # NBP2-81098JF549

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Janelia Fluor 549

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # H22
View all Fc gamma RI/CD64 Primary Antibodies »

Product Specifications

Immunogen

This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human Fc gamma RI/CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.

Specificity

This immunotoxin is specific for human Fc gamma RI/CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Applications for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 549]

Application
Recommended Usage

ELISA

Validated for Competitive ELISA
Application Notes
Optimal dilution of this antibody should be experimentally determined. This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fc gamma RI/CD64

CD64 is a 72 kD single chain type I glycoprotein also known as Fc gammaRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN- gamma stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Long Name

Fc gamma Receptor I

Alternate Names

CD64, CD64a, FCGR1, FcgRI, FcgRIA, FCRI, FcRIA

Gene Symbol

FCGR1A

Additional Fc gamma RI/CD64 Products

Product Documents for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 549]

Certificate of Analysis

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Product Specific Notices for Fc gamma RI/CD64 Antibody (H22) [Janelia Fluor® 549]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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