Fc gamma RII/CD32 Antibody (AT10) - BSA Free

Novus Biologicals | Catalog # NB100-65338

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Porcine

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation, Functional

Cited:

Flow Cytometry, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AT10

Format

BSA Free
View all Fc gamma RII/CD32 Primary Antibodies »

Product Specifications

Immunogen

K562 cell line

Reactivity Notes

Predicted cross-reactivities: Rhesus Monkey, Dog, Porcine

Specificity

NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fc gamma RII/CD32 Antibody (AT10) - BSA Free

Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]

Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]

Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]

Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) - BSA Free [NB100-65338]

Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the Biotin conjugate of NB100-65338. Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.

Applications for Fc gamma RII/CD32 Antibody (AT10) - BSA Free

Application
Recommended Usage

Flow Cytometry

20 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:500-1:1000

Immunoprecipitation

20 ug/ml

Reviewed Applications

Read 1 review rated 4 using NB100-65338 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fc gamma RII/CD32

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Long Name

Fc gamma Receptor II

Alternate Names

FcgRII

Gene Symbol

FCGR2B

Additional Fc gamma RII/CD32 Products

Product Documents for Fc gamma RII/CD32 Antibody (AT10) - BSA Free

Certificate of Analysis

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Product Specific Notices for Fc gamma RII/CD32 Antibody (AT10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fc gamma RII/CD32 Antibody (AT10) - BSA Free

Customer Reviews for Fc gamma RII/CD32 Antibody (AT10) - BSA Free (1)

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  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: Human
    Species: Human
    Verified Customer | Posted 11/16/2011

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