Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-70709
![Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5)Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Western-Blot-NBP2-70709-img0001.jpg "Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5)Azide and BSA Free [NBP2-70709]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI9G5
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
34.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free
Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5)Azide and BSA Free [NBP2-70709]
Western Blot: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.![Immunocytochemistry/ Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70709-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunocytochemistry/ Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunocytochemistry/Immunofluorescence: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0010.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human prostate tissue using anti-FCGR2A mouse monoclonal antibody.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0003.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FCGR2A mouse monoclonal antibody.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0004.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human lung tissue using anti-FCGR2A mouse monoclonal antibody.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0005.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human breast tissue using anti-FCGR2A mouse monoclonal antibody.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0006.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human colon tissue using anti-FCGR2A mouse monoclonal antibody.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0007.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human endometrium tissue using anti-FCGR2A mouse monoclonal antibody.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0008.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human Kidney tissue using anti-FCGR2A mouse monoclonal antibody.![Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70709-img0009.jpg "Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunohistochemistry: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Staining of paraffin-embedded Human pancreas tissue using anti-FCGR2A mouse monoclonal antibody.![Immunoprecipitation: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]](https://resources.rndsystems.com/images/products/Fc-gamma-RIIA-CD32a-Antibody-OTI9G5-Azide-and-BSA-Free-Immunoprecipitation-NBP2-70709-img0011.jpg "Immunoprecipitation: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]")
Immunoprecipitation: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709]
Immunoprecipitation: Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free [NBP2-70709] - Lane 1: Negative Control Lane 2: NBP1-47738 Lane 3: NBP1-47739 Lane 4: NBP1-47740 Lane 5: NBP1-47741 Lane 6: NBP1-47742 Lane 7: NBP1-47734 Lane 8: NBP1-47735 Lane 9: NBP1-47736 Lane 10: NBP1-47737 Lane 11: NBP1-47743 Lane 12: NBP1-47744Applications for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50
Immunoprecipitation
2ug/500ul
Western Blot
1:200 - 1:1000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: Fc gamma RIIA/CD32a
Long Name
Fc gamma Receptor II A
Alternate Names
CD32a, FCGR2A, FcgRIIA, FCRIIA
Gene Symbol
FCGR2A
Additional Fc gamma RIIA/CD32a Products
Product Documents for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Fc gamma RIIA/CD32a Antibody (OTI9G5) - Azide and BSA Free
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Q: Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
A: For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.
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