Ferritin Heavy Chain Antibody (HL1905)

Novus Biologicals | Catalog # NBP3-48666

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Canine, Feline

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1905
View all Ferritin Heavy Chain Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Ferritin Heavy Chain.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ferritin Heavy Chain Antibody (HL1905)

Ferritin Heavy Chain Antibody (HL1905)

Western Blot: Ferritin Heavy Chain Antibody (HL1905) [NBP3-48666] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Ferritin Heavy Chain Antibody (HL1905)

Western Blot: Ferritin Heavy Chain Antibody (HL1905) [NBP3-48666] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Ferritin Heavy Chain Antibody (HL1905)

Western Blot: Ferritin Heavy Chain Antibody (HL1905) [NBP3-48666] -

Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 15% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Ferritin Heavy Chain Antibody (HL1905)

Western Blot: Ferritin Heavy Chain Antibody (HL1905) [NBP3-48666] -

Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Ferritin Heavy Chain antibody [HL1905] (NBP3-48666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Ferritin Heavy Chain Antibody (HL1905)

Application
Recommended Usage

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ferritin Heavy Chain

Stores iron in a soluble. nontoxic. readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Ferritin is a ubiquitous and highly conserved protein which plays a major role in iron homeostasis by sequestering and storing iron in a non-toxic and soluble form. It forms a holoenzyme of ~450 kDa, consisting of 24 subunits of two types, H (heavy; 21 kDa) and L (light; 19 kDa), and is capable of storing up to 4,500 atoms of ferric iron. Depending on the tissue type and physiological status of the cell, the ratio of H to L subunits in ferritin can vary widely. Ferritin is found in the liver, spleen, kidney and heart, with smaller amounts being found in blood. Serum ferritin levels serve as an indicator of the amount of iron stored in the body. Serum ferritin is the most sensitive test for anaemia, and is also used as a marker for restless leg syndrome, hemochromatosis and porphyria. As ferritin is an acute-phase reactant, it is often elevated during infection. Defects in ferritin proteins are associated with several neurodegenerative diseases.

Alternate Names

apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, PIG15, placenta immunoregulatory factor, PLIF, proliferation-inducing protein 15

Gene Symbol

FTH1

Additional Ferritin Heavy Chain Products

Product Documents for Ferritin Heavy Chain Antibody (HL1905)

Certificate of Analysis

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Product Specific Notices for Ferritin Heavy Chain Antibody (HL1905)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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