Ferroportin/SLC40A1 Antibody [PE]

Novus Biologicals | Catalog # NBP1-21502PE

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Polyclonal Rabbit IgG
View all Ferroportin/SLC40A1 Primary Antibodies »

Product Specifications

Immunogen

This Ferroportin/SLC40A1 Antibody was developed against a synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot: Q9NP59]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24895335).

Localization

Cell membrane (high), Cytoplasm (low)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ferroportin/SLC40A1 Antibody [PE]

Flow Cytometry Analysis of B Lymphocytes Stained with Phycoerythrin Conjugated Ferroportin/SLC40A1 Antibody

Flow Cytometry Analysis of B Lymphocytes Stained with Phycoerythrin Conjugated Ferroportin/SLC40A1 Antibody

Analysis of Ferroportin in human B lymphocytes in peripheral blood using PE conjugated anti-Ferroportin antibody. Image from verified customer review.

Applications for Ferroportin/SLC40A1 Antibody [PE]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-21502PE in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Ferroportin/SLC40A1

Ferroportin is a 12-transmembrane domain protein, belonging to the major facilitator superfamily of transporters of small molecules, that is localized to the plasma membrane. Human Ferroportin has a theoretical molecular weight of 62.5 kDa. Ferroportin (FPN1 or SLC40A1) functions as an iron-regulated transporter (highly expressed in placenta, intestine, muscle, spleen, macrophages etc.) and is the receptor for the iron-regulatory hormone, hepcidin. In iron metabolism, FPN1 plays a key role in intestinal iron absorption as well as cellular iron release and mediates iron absorption in the presence of ferroxidases, hephaestin (HP) and/or ceruloplasmin (CP). FPN1 is implicated in iron export from duodenal epithelial cells and in the transfer of iron between maternal and fetal circulation. FPN1 transports iron in the ferrous form whereas plasma transferrin only binds iron's ferric form. Ferroxidases are key players in oxidizing iron transported by FPN1 and without the activity of ferroxidases, FPN1 is internalized followed by degradation. While other cell types utilize the circulating or GPI-linked multicopper ferroxidase CP for FPN1, intestinal cells utilize a membrane-bound HP, a paralog of CP that also show interaction with FPN1 (1).

FPN1 regulation is dependent on the cell type and involves transcriptional, posttranscriptional, and posttranslational mechanisms including hepcidin-mediated endocytosis and proteolysis. Hepcidin controls the concentration of FPN1 in the membrane, with hepcidin deficiency resulting in iron overload (high iron) and hepcidin excess leading to iron restriction and anemia (2). Ferroportin disease or hemochromatosis type 4 (HFE4) is associated with distinct FPN1 variants with either reduced FPN1 cell surface expression/iron export capacity or hepcidin resistance and iron overload (3, 4).

References

1. De Domenico I, Ward DM, Kaplan J. (2011) Hepcidin and ferroportin: the new players in iron metabolism. Semin Liver Dis. 31(3):272-9. PMID: 21901657

2. Drakesmith H, Nemeth E, Ganz T. (2015) Ironing out Ferroportin. Cell Metab. 22(5):777-87. PMID: 26437604

3. Pietrangelo A. (2017) Ferroportin disease: pathogenesis, diagnosis and treatment. Haematologica. 102(12):1972-1984. PMID: 29101207

4. Vlasveld LT, Janssen R, Bardou-Jacquet E, Venselaar H, Hamdi-Roze H, Drakesmith H, Swinkels DW. (2019) Twenty Years of Ferroportin Disease: A Review or An Update of Published Clinical, Biochemical, Molecular, and Functional Features. Pharmaceuticals (Basel). 12(3). pii: E132. PMID: 31505869

Long Name

Solute Carrier Family 40 Member 1

Alternate Names

FPN1, HFE4, IREG1, MST079, MTP1, SLC11A3, SLC40A1

Gene Symbol

SLC40A1

Additional Ferroportin/SLC40A1 Products

Product Documents for Ferroportin/SLC40A1 Antibody [PE]

Certificate of Analysis

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Product Specific Notices for Ferroportin/SLC40A1 Antibody [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ferroportin/SLC40A1 Antibody [PE]

Customer Reviews for Ferroportin/SLC40A1 Antibody [PE] (1)

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Customer Images

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  • Name: Bill Merritt
    Application: Flow Cytometry
    Sample Tested: B lymphocytes in peripheral blood
    Species: Human
    Verified Customer | Posted 02/19/2014
    Ferroportin/SLC40A1 Antibody [PE] NBP1-21502PE

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