FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF-19 subfamily members lack a heparin binding domain, enabling them to freely circulate as endocrine factors and diffuse within tissues. FGF-21 signals through Klotho beta in complex with FGF receptors. It regulates cellular metabolism including glucose uptake in adipocytes and cellular sensitivity to insulin. It is basally expressed in the pancreas, thymus, liver, and adipose tissue and is upregulated in liver, muscle, and fat in obesity and diabetes. FGF-21 also plays a role in cell survival and proliferation, mesenchymal stem cell differentiation, circadian rhythm, and controlling reproductive capacity.
Monkey FGF-21 ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-18048
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Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
18.75 pg/mL
Assay Range
31.25-2000 pg/mL
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Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Monkey
Precision
Intra-Assay Precision (Precision within an assay) %CV 6.32
Inter-Assay Precision (Precision between assays) %CV 6.81
Recovery for Monkey FGF-21 ELISA Kit (Colorimetric)
Recovery
84-107%
Linearity
Scientific Data Images for Monkey FGF-21 ELISA Kit (Colorimetric)
ELISA: Monkey FGF-21 ELISA Kit (Colorimetric) [NBP3-18048] -
ELISA: Monkey FGF-21 ELISA Kit (Colorimetric) [NBP3-18048] - Standard Curve ReferenceKit Contents for Monkey FGF-21 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: FGF-21
Long Name
Fibroblast Growth Factor 21
Alternate Names
FGF21
Gene Symbol
fgf21
Additional FGF-21 Products
Product Documents for Monkey FGF-21 ELISA Kit (Colorimetric)
Product Specific Notices for Monkey FGF-21 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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