FGF-23 (fibroblast growth factor 23) is one of three FGF family members that appear to play a role in endocrine metabolism. It is described as being a phosphatonin, a molecule that reduces circulating plasma phosphate. FGF-23 does this, in part, by blocking Vitamin D activation. Active Vitamin D promotes intestinal phosphate absorption.
FGF-23 Antibody (FGF23/4168)
Novus Biologicals | Catalog # NBP3-07378
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry-Paraffin, Protein Array
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2c Kappa Clone # FGF23/4168
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Product Specifications
Immunogen
Recombinant fragment (around aa25-251) of human FGF-23 protein (exact sequence is proprietary) (Uniprot: Q9GZV9)
Localization
Secreted (extracellular)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2c Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08383)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for FGF-23 Antibody (FGF23/4168)
![Immunohistochemistry-Paraffin: FGF-23 Antibody (FGF23/4168) [NBP3-07378]](https://beta-resources.rndsystems.com/images/products/FGF-23-Antibody-FGF23-4168-Immunohistochemistry-Paraffin-NBP3-07378-img0002.jpg "Immunohistochemistry-Paraffin: FGF-23 Antibody (FGF23/4168) [NBP3-07378]")
Immunohistochemistry-Paraffin: FGF-23 Antibody (FGF23/4168) [NBP3-07378]
Immunohistochemistry-Paraffin: FGF-23 Antibody (FGF23/4168) [NBP3-07378] - Formalin-fixed, paraffin-embedded human kidney stained with FGF-23 Mouse Monoclonal Antibody (FGF-23/4168).![Protein Array: FGF-23 Antibody (FGF23/4168) [NBP3-07378]](https://beta-resources.rndsystems.com/images/products/FGF-23-Antibody-FGF23-4168-Protein-Array-NBP3-07378-img0001.jpg "Protein Array: FGF-23 Antibody (FGF23/4168) [NBP3-07378]")
Protein Array: FGF-23 Antibody (FGF23/4168) [NBP3-07378]
Protein Array: FGF-23 Antibody (FGF23/4168) [NBP3-07378] - Analysis of Protein Array containing more than 19,000 full-length human proteins using FGF-23 Mouse Monoclonal Antibody (FGF-23/4168).Applications for FGF-23 Antibody (FGF23/4168)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: FGF-23
Additional FGF-23 Products
Product Documents for FGF-23 Antibody (FGF23/4168)
Product Specific Notices for FGF-23 Antibody (FGF23/4168)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for FGF-23 Antibody (FGF23/4168)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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