FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.
FGFR2 Antibody (98742) [DyLight 594]
Novus Biologicals | Catalog # FAB6842M
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
DyLight 594 (Excitation = 593 nm, Emission = 618 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 98742
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human FGF R2 isoforms and Mouse myeloma cell line NS0-derived recombinant human FGF R2 isoforms
Specificity
Detects all isoforms of human FGF R2 in direct ELISAs and Western blots. Does not cross‑react with any isoform of mouse FGF R2 or human FGF R1, FGF R3, or FGF R4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for FGFR2 Antibody (98742) [DyLight 594]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FGFR2
Long Name
Fibroblast Growth Factor Receptor 2
Alternate Names
CD332, FGF R2
Gene Symbol
FGFR2
Additional FGFR2 Products
Product Documents for FGFR2 Antibody (98742) [DyLight 594]
Product Specific Notices for FGFR2 Antibody (98742) [DyLight 594]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
