FGFR2 Antibody - Humanized, (Research Grade bemarituzumab Biosimilar) - Low Endotoxin, Azide and BSA Free

Novus Biologicals | Catalog # NBP3-28356

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 expressed in CHO

Format

Low Endotoxin, Azide and BSA Free
View all FGFR2 Primary Antibodies »

Product Specifications

Immunogen

FGFR2 / CD332

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.24 kDa.

Also known as 'bemarituzumab'.

This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Scientific Data Images

FGFR2 Antibody (bemarituzumab) - Humanized

Functional: FGFR2 Antibody (bemarituzumab) - Humanized[NBP3-28356] -

FGFR2 Antibody (bemarituzumab) - Humanized -ADCC luciferase Assay on KATOIII cells. The maximum suppression factor is approximately 14.
FGFR2 Antibody (bemarituzumab) - Humanized

Functional: FGFR2 Antibody (bemarituzumab) - Humanized[NBP3-28356] -

FGFR2 Antibody (bemarituzumab) - Humanized induced ADCC activity was evaluated using Human FGFR2 HEK293 Reporter Cell.The max induction fold was approximately 25.
FGFR2 Antibody (bemarituzumab) - Humanized

In vivo assay: FGFR2 Antibody (bemarituzumab) - Humanized[NBP3-28356] -

Bemarituzumab inhibited the tumor growth of SNU-16 on Balb/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 29.7% at 2 mpk at D62.
FGFR2 Antibody (bemarituzumab) - Humanized

Flow Cytometry: FGFR2 Antibody (bemarituzumab) - Humanized[NBP3-28356] -

SNU-16 cells were stained with FGFR2 Antibody (bemarituzumab) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.5368 ug/mL.

Applications

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Format

Low Endotoxin, Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR2

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGFR1 through 5. FGFRs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGFR1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGFR5 does not. Multiple forms of FGFR1, 2, and 3 are generated by alternative splicing.

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Gene Symbol

FGFR2

UniProt

P21802

Additional FGFR2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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