Fibrillarin Antibody

Novus Biologicals | Catalog # NBP2-26151

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Fibrillarin Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CRGKEDALVTKN corresponding to internal region according to NP_001427.2.

Marker

Nucleolar Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Fibrillarin Antibody

Western Blot: Fibrillarin Antibody [NBP2-26151]

Western Blot: Fibrillarin Antibody [NBP2-26151]

Western Blot: Fibrillarin Antibody [NBP2-26151] - NBP2-26151 (0.1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Fibrillarin Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 34 kDa band observed in HEK293 lysates (calculated MW of 33.8 kDa band according to NP_001427.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fibrillarin

Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologous to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended beta-sheets flanked by 4 alpha-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.

Alternate Names

EC 2.1.1,34-kD nucleolar scleroderma antigen, EC 2.1.1.-, EC 2.1.1.37, FIB, FIB1,34 kDa nucleolar scleroderma antigen, fibrillarin, FLRNrRNA 2'-O-methyltransferase fibrillarin, RNA, U3 small nucleolar interacting protein 1, RNU3IP1

Entrez Gene IDs

2091 (Human); 14113 (Mouse); 292747 (Rat)

Gene Symbol

FBL

Additional Fibrillarin Products

Product Documents for Fibrillarin Antibody

Certificate of Analysis

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Product Specific Notices for Fibrillarin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Fibrillarin Antibody

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