Fibrinopeptide A Antibody (1D12) - Azide and BSA Free

Novus Biologicals | Catalog # H00002243-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D12

Format

Azide and BSA Free
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Product Specifications

Immunogen

FGA (AAH20764, 1 a.a. ~ 218 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MFSMSIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS

Specificity

FGA - fibrinogen alpha chain (1D12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Fibrinopeptide A Antibody (1D12) - Azide and BSA Free

Western Blot: Fibrinopeptide A Antibody (1D12) [H00002243-M01]

Western Blot: Fibrinopeptide A Antibody (1D12) [H00002243-M01]

Western Blot: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Analysis of FGA expression in transfected 293T cell line by FGA monoclonal antibody (M01), clone 1D12.Lane 1: FGA transfected lysate(24.7 KDa).Lane 2: Non-transfected lysate.
Proximity Ligation Assay: Fibrinopeptide A Antibody (1D12) [H00002243-M01]

Proximity Ligation Assay: Fibrinopeptide A Antibody (1D12) [H00002243-M01]

Proximity Ligation Assay: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Analysis of protein-protein interactions between APOA1 and FGA. HeLa cells were stained with anti-APOA1 rabbit purified polyclonal 1:1200 and anti-FGA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: Fibrinopeptide A Antibody (1D12) [H00002243-M01]

Immunoprecipitation: Fibrinopeptide A Antibody (1D12) [H00002243-M01]

Immunoprecipitation: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Analysis of FGA transfected lysate using anti-FGA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGA MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Fibrinopeptide A Antibody (1D12) [H00002243-M01] - Detection limit for recombinant GST tagged FGA is 1 ng/ml as a capture antibody.

Applications for Fibrinopeptide A Antibody (1D12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fibrinogen A

The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia and renal amyloidosis. Alternative splicing results in two isoforms which vary in the carboxy-terminus. [provided by RefSeq]

Alternate Names

FGA, Fib2, Fibrinogen alpha chain, Fibrinogen alpha subunit, Fibrinogen Type I, FPA

Entrez Gene IDs

2243 (Human)

Gene Symbol

FGA

Additional Fibrinogen A Products

Product Documents for Fibrinopeptide A Antibody (1D12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fibrinopeptide A Antibody (1D12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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