Ficolin-1 Antibody (7G1)

Novus Biologicals | Catalog # NBP3-11282

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoassay, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7G1
View all Ficolin-1 Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant Ficolin-1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Ficolin-1 Antibody (7G1)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Immunoassays and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Ficolin-1

The monoclonal antibody 7G1 recognizes human M-ficolin, also known as ficolin-1. Ficolins are a family of pattern recognition molecules containing both a collagen-like domain and a fibrinogen-like domain that are capable of activating the complement system, through association with MASPs, on the surface of microorganisms. In human, three Ficolins are known: M-Ficolin, L- Ficolin (Ficolin-2), and H-Ficolin (Ficolin-3). M-Ficolin, is mainly expressed by monocytes, granulocytes and lung cells and is present locally in areas of inflammation. However, it was recently also found in serum suggesting that M-Ficolin also has a role in systemic immunity like L- and H-Ficolin. Functional analysis of M-Ficolin has demonstrated that it's ligand is sialic acid present on bacteria since binding was abolished by prior treatment of the bacteria with sialidase. Furthermore, it was shown that M-ficolin facilitates the uptake of E. coli- by a leukemic monocyte lymphoma cell line, suggesting that it functions as a phagocytic receptor on the surface of circulating monocytes. Low cord blood concentration of M-ficolin has been- associated with higher necrotizing enterocolitis-associated fatality and two identified SNPs in the M-ficolin gene are associated with the development of rheumatoid arthritis. The monoclonal antibody 7G1 is suitable for use in immunoassays and western blotting.

Long Name

Ficolin [Collagen/Fibrinogen Domain Containing] 1

Alternate Names

FCN1, Fcna, FCNM, Ficolin1

Gene Symbol

FCN1

Additional Ficolin-1 Products

Product Documents for Ficolin-1 Antibody (7G1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ficolin-1 Antibody (7G1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

C-type Lectin Family

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Protocols

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