FITC is a small organic fluorochrome dye commonly used for a variety of labeling applications such as conjugation to antibodies or other proteins. Excitation of FITC results in emission of a visible yellow-green signal.
FITC Antibody (6R1B5)
Novus Biologicals | Catalog # NBP3-16080
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6R1B5 expressed in HEK293
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Product Specifications
Immunogen
FITC
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FITC Antibody (6R1B5)
Western Blot: FITC Antibody (6R1B5) [NBP3-16080] -
Western Blot: FITC Antibody (6R1B5) [NBP3-16080] - Westemn blot analysis of recombiant FITC conjugated goat IG protein using FITC Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for FITC Antibody (6R1B5)
Application
Recommended Usage
ELISA
1:500 - 1:2000
Western Blot
1:1000 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FITC
Long Name
Fluorescein Isothiocyanate
Alternate Names
Fluorescein Isothiocyanate
Additional FITC Products
Product Documents for FITC Antibody (6R1B5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FITC Antibody (6R1B5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for FITC Antibody (6R1B5)
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Q: What is the molecular weight of Fluorescein Isothiocyanate (FITC)?
A: The molecular weight of FITC is 389.38 Daltons. However, R&D Systems uses 5- (and 6-) Carboxyfluorescein (CFS) for antibody conjugation, which has a molecular weight of 473 Daltons.
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