Key Product Details

Species Reactivity

Non-species specific

Applications

Validated:

Western Blot, ELISA, Fluorophore-linked immunosorbent assay, Flow Cytometry

Cited:

Western Blot

Label

Alkaline Phosphatase

Antibody Source

Polyclonal Sheep IgG
View all FITC Primary Antibodies »

Product Specifications

Immunogen

Pooled antisera from sheep hyperimmunized with fluorescein isothiocyanate (Isomer I)

Specificity

Recognizes Fluorescein isothiocyanate. Cross Adsorption None; may react with other fluorescein derivatives

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for FITC Antibody [Alkaline Phosphatase]

Fluorophore-linked immunosorbent assay: FITC Antibody [Alkaline Phosphatase] [NB100-64618] - FLISA plate was coated with Goat Anti-Human IgG-FITC and Goat Anti-Human IgG-BIOT. Antibodies were detected with serially diluted Sheep Anti-FITC-PE.

Applications for FITC Antibody [Alkaline Phosphatase]

Application
Recommended Usage

ELISA

1:2000-1:4000

Fluorophore-linked immunosorbent assay

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
WB reactivity reported in (PMID: 24166503).

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50 mM Tris (pH 8.0), 1 mM MgCl2, and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FITC

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody [Alkaline Phosphatase]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FITC Antibody [Alkaline Phosphatase]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for FITC Antibody [Alkaline Phosphatase]

Customer Reviews for FITC Antibody [Alkaline Phosphatase]

There are currently no reviews for this product. Be the first to review FITC Antibody [Alkaline Phosphatase] and earn rewards!

Have you used FITC Antibody [Alkaline Phosphatase]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FITC Antibody [Alkaline Phosphatase]

Showing  1 - 2 of 2 FAQs Showing All

  • Q: How many alkaline phosphatases are bound to one IgG? And I want to know the labeling method. What is the IgG site crosslinking alkaline phosphatase? NH2 or SH site?

    A: We do not measure the amount of AP bound to the immunoglobulins. We conjugate the antibody with an over-saturation of AP which may result in free AP in the product. The AP binds to cysteine.

  • Q: What is the molecular weight of Fluorescein Isothiocyanate (FITC)?

    A: The molecular weight of FITC is 389.38 Daltons. However, R&D Systems uses 5- (and 6-) Carboxyfluorescein (CFS) for antibody conjugation, which has a molecular weight of 473 Daltons.

  • Q: How many alkaline phosphatases are bound to one IgG? And I want to know the labeling method. What is the IgG site crosslinking alkaline phosphatase? NH2 or SH site?

    A: We do not measure the amount of AP bound to the immunoglobulins. We conjugate the antibody with an over-saturation of AP which may result in free AP in the product. The AP binds to cysteine.

  • Q: What is the molecular weight of Fluorescein Isothiocyanate (FITC)?

    A: The molecular weight of FITC is 389.38 Daltons. However, R&D Systems uses 5- (and 6-) Carboxyfluorescein (CFS) for antibody conjugation, which has a molecular weight of 473 Daltons.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Antibodies