Found in Inflammatory Zone 1 (FIZZ1), also known as Resistin-like Molecule (RELM) alpha, is a cysteine-rich secreted protein that is a member of the FIZZ/RELM family. It is 111 amino acids (aa) in length, with aa 1-23 serving as a putative signal peptide. Mouse FIZZ1/RELM alpha has a predicted molecular weight of approximately 9.5 kDa, and shares 50% aa sequence identity with the human ortholog. FIZZ1/RELM alpha is expressed in white adipose tissue, but not in preadipocytes nor 3T3-L1 adipocytes. It is also expressed in lung tissue, and its expression has been seen to increase during allergic pulmonary inflammation. FIZZ1/RELM alpha has also been detected in the mammary glands, heart, tongue, skeletal muscles, and alternatively-activated macrophages.
FIZZ1/RELM alpha Antibody (226033) [Allophycocyanin]
Novus Biologicals | Catalog # FAB1523A
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 226033
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Product Specifications
Immunogen
E. coli-derived recombinant mouse FIZZ1/RELM alpha
Asp24-Ser111
Accession # Q9EP95
Asp24-Ser111
Accession # Q9EP95
Specificity
Detects mouse FIZZ1/RELM alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) RELM beta or rmResistin is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for FIZZ1/RELM alpha Antibody (226033) [Allophycocyanin]
Application
Recommended Usage
Immunocytochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: FIZZ1/RELM alpha
Long Name
Found in Inflammatory Zone 1/Resistin-like Molecule alpha
Alternate Names
RELM alpha, RETNLA, RETNLB
Gene Symbol
RETNLB
Additional FIZZ1/RELM alpha Products
Product Documents for FIZZ1/RELM alpha Antibody (226033) [Allophycocyanin]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for FIZZ1/RELM alpha Antibody (226033) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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