Mouse FIZZ1/RELM alpha Antibody

Catalog # Availability Size / Price Qty
MAB1523-SP
MAB1523-500
MAB1523-100
FIZZ1/RELM alpha  in BV-2 Mouse Cell Line.
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Product Details
Citations (4)
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Mouse FIZZ1/RELM alpha Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FIZZ1/RELM alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) RELM beta or rmResistin is observed.
Source
Monoclonal Rat IgG2A Clone # 226033
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse FIZZ1/RELM alpha
Asp24-Ser111
Accession # Q9EP95
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse FIZZ1/RELM alpha
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry FIZZ1/RELMa antibody in BV-2 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

FIZZ1/RELM alpha in BV-2 Mouse Cell Line. FIZZ1/RELMa was detected in immersion fixed BV-2 mouse microglia cell line using Rat Anti-Mouse FIZZ1/RELMa Monoclonal Antibody (Catalog # MAB1523) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FIZZ1/RELM alpha

RELM alpha, also known as ‘found in inflammatory zone 1’ (FIZZ1) belongs to the RELM/FIZZ family of cysteine rich secretory proteins. RELM alpha is most abundantly expressed in white adipose tissue but not in preadipocytes or 3T3-L1 adipocytes. RELM alpha expression is also detected in mammary tissue, heart, lung, and tongue.

Long Name
Found in Inflammatory Zone 1/Resistin-like Molecule alpha
Entrez Gene IDs
84666 (Human); 57262 (Mouse)
Alternate Names
1810019L16Rik; FIZZ1; Fizz-1; HIMF; RELM alpha; RELMa; RELMalpha; RELM-alpha; Retnla resistin like alpha; RETNLA; RETNLB; Xcp2

Product Datasheets

Citations for Mouse FIZZ1/RELM alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Epigenetic stabilization of DC and DC precursor classical activation by TNFalpha contributes to protective T cell polarization
    Authors: AJ Eastman, J Xu, J Bermik, N Potchen, A den Dekker, LM Neal, G Zhao, A Malachowsk, M Schaller, S Kunkel, JJ Osterholze, I Kryczek, MA Olszewski
    Sci Adv, 2019;5(12):eaaw9051.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Fluorescence Microscopy
  2. Concerted IL-25R and IL-4R? signaling drive innate type 2 effector immunity for optimal helminth expulsion
    Authors: KA Smith, S Löser, F Varyani, Y Harcus, HJ McSorley, AN McKenzie, RM Maizels
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
    Applications: Flow Cytometry
  3. Role of arginase 1 from myeloid cells in th2-dominated lung inflammation.
    Authors: Barron L, Smith A, El Kasmi K, Qualls J, Huang X, Cheever A, Borthwick L, Wilson M, Murray P, Wynn T
    PLoS ONE, 2013;8(4):e61961.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice.
    Authors: Heneka M, Kummer M, Stutz A, Delekate A, Schwartz S, Vieira-Saecker A, Griep A, Axt D, Remus A, Tzeng T, Gelpi E, Halle A, Korte M, Latz E, Golenbock D
    Nature, 2013;493(7434):674-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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