FIZZ1/RELM alpha in BV-2 Mouse Cell Line.
FIZZ1/RELM alpha was detected in immersion fixed BV-2 mouse microglia cell line using Rat Anti-Mouse FIZZ1/RELM alpha Monoclonal Antibody (Catalog # MAB1523) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (orange; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FIZZ1/RELM alpha
RELM alpha, also known as ‘found in inflammatory zone 1’ (FIZZ1) belongs to the RELM/FIZZ family of cysteine rich secretory proteins. RELM alpha is most abundantly expressed in white adipose tissue but not in preadipocytes or 3T3-L1 adipocytes. RELM alpha expression is also detected in mammary tissue, heart, lung, and tongue.
Found in Inflammatory Zone 1/Resistin-like Molecule alpha
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