Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-12311

Novus Biologicals
Discontinued Product
NBP2-12311 has been discontinued. View all Flt-3/Flk-2 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0294-9G23

Format

Azide and BSA Free
View all Flt-3/Flk-2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant Flt-3/Flk-2/CD135

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

Western Blot: Flt-3/Flk-2/CD135 Antibody (MM0294-9G23)Azide and BSA Free [NBP2-12311]

Western Blot: Flt-3/Flk-2/CD135 Antibody (MM0294-9G23)Azide and BSA Free [NBP2-12311]

Western Blot: Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) [NBP2-12311] - Lysates of human placental tissue.

Applications for Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

Application
Recommended Usage

Simple Western

1:100

Western Blot

1:500-1:1000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in CAL-62, CHO, EoL-1, HL-60, Huh-7, MOLM-13, MV4-11, NCI-H1975, NUGC-4, Bone marrow, separated by Size, antibody dilution of 1:100, apparent MW was 130 kDa

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Flt-3/Flk-2

Stem cell tyrosine kinase (STK-1) has been cloned from a CD34+ hematopoietic stem cell enriched library and identified as the human homolog of a previously identified gene of mouse origin designated either Flk-2 or Flt-3. The STK-1 cDNA encodes a protein of 993 amino acids with 85% identity to Flt-3/Flk-2. STK-1 is a member of the type III receptor tyrosine kinase family that includes Kit (steel factor receptor), Fms and PDGF. STK-1 expression in blood and marrow is restricted to CD34+ cells, a population greatly enriched for hematopoietic stem/progenitor cells. STK-1 antiserum recognizes two polypeptides in these cells. The mouse homolog of STK-1, designated Flt-3/ Flk-2, is expressed at high levels in hematopoietic cells and also in neural, gonadal, hepatic and placental tissues. It has been suggested that STK-1 and its murine homolog Flt-3/ Flk-2 may function as growth factor receptors on hematopoietic stem and/or progenitor cells.

Long Name

fms-like Tyrosine Kinase 3

Alternate Names

CD135, Flk-2, Flt3, STK-1

Gene Symbol

FLT3

UniProt

P36888

Additional Flt-3/Flk-2 Products

Product Documents for Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

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FAQs for Flt-3/Flk-2/CD135 Antibody (MM0294-9G23) - Azide and BSA Free

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  • Q: We have purchased your Flt3/CD135 Antibody (MM0294-9G23) cat# NBP2-12311 (0.1 mg lyophilized in PBS). The reconstitution instructions on the data sheet say Please contact technical support for detailed reconstitution instructions. Can you please give me the instructions how I should correctly resuspend this reagent? Also, can you confirm that this reagent is carrier free and is only the Ab in PBS (i.e. no BSA)?

    A: You should spin down the vial and reconstitute in 200uL of PBS. This antibody contains no carrier proteins.

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