Q: We received an inquiry about Folic acid ELISA Kit, Cat# NBP2-59996. The customer would like to know the plate type in this kit. Could you tell us whether the assay plate is strip type (8×12 well) or one strip 96-well type?

A: These are 96 well plates and yes, the plate can be divided into the 8x12 strips.

Q: We want to know the plate type in your Folic acid ELISA Kit (NBP2-59996). Could you tell us whether the assay plate is strip type (8×12 well) or one strip 96-well type?

A: The plate of this product can be divided into the 8x12 strips.

Mouse Folic acid ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-59996

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Product Specifications
Precision
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

0.195 ng/mL

Assay Range

0.195 ng/mL - 50 ng/mL

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Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Mouse

Specificity

This assay has high sensitivity and excellent specificity for detection of mouse FA. No significant cross-reactivity or interference between mouse FA and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Mouse Folic acid ELISA Kit (Colorimetric)

ELISA: Mouse Folic acid ELISA Kit (Colorimetric) [NBP2-59996]

ELISA: Mouse Folic acid ELISA Kit (Colorimetric) [NBP2-59996] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Mouse Folic acid ELISA Kit (Colorimetric) [NBP2-59996] -

Mouse Folic acid ELISA Kit (Colorimetric) [NBP2-59996] - Standard Curve Reference

Kit Contents for Mouse Folic acid ELISA Kit (Colorimetric)

Adhesive Strip (For 96 wells)
Antibody (100 x concentrate)
Antibody Diluent
Assay plate
HRP-conjugate (100 x concentrate)
HRP-conjugate Diluent
Instruction manual
Sample Diluent
Standard (Freeze dried)
Stop Solution
TMB Substrate
Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Folic acid

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are added to the appropriate microtiter plate wells with antibody specific for FA and Horseradish Peroxidase (HRP) conjugated goat-anti-rabbit antibody. The competitive inhibition reaction is launched between with pre-coated FA and FA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of FA in the sample. The color development is stopped and the intensity of the color is measured.

Alternate Names

Folate, Tetrahydrofolate, Tetrahydrofolic acid, THFA

Additional Folic acid Products

Product Documents for Mouse Folic acid ELISA Kit (Colorimetric)

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Download Manual - Mouse Folic acid ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Mouse Folic acid ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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