FoxC2 Antibody

Novus Biologicals | Catalog # NB100-1269

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow Cytometry

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence RHAAPYSYDCTKY corresponding to C-Terminus according to NP_005242.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Goat FoxC2 Antibody (NB100-1269) is a polyclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. Anti-FoxC2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FoxC2 Antibody

Western Blot: FoxC2 Antibody [NB100-1269]

Western Blot: FoxC2 Antibody [NB100-1269]

Western Blot: FoxC2 Antibody [NB100-1269] - Staining of nuclear HEK293 cell lysate with antibody at 2 ug/mL (A) and negative control human pancreas cell lysate with antibody at 1 ug/mL (B) (RIPA buffer 35 ug total protein per lane). Detected by chemiluminescence.
Flow Cytometry: FoxC2 Antibody [NB100-1269]

Flow Cytometry: FoxC2 Antibody [NB100-1269]

Flow Cytometry: FoxC2 Antibody [NB100-1269] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for FoxC2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

2 ug/mL
Application Notes
IHC validated for previous lots. Use in IHC-frozen, and ICC/IF reported in scientific literature (PMID: 23862012).

Reviewed Applications

Read 2 reviews rated 4.5 using NB100-1269 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FoxC2

FOXC2 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

Long Name

Forkhead Box C2

Alternate Names

Fkh14, LD, MFH1

Entrez Gene IDs

2303 (Human); 14234 (Mouse)

Gene Symbol

FOXC2

UniProt

Additional FoxC2 Products

Product Documents for FoxC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FoxC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FoxC2 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images


Showing  1 - 2 of 2 reviews Showing All
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Human Embryonic Kidneys Week 17.1
    Species: Human
    Verified Customer | Posted 07/22/2016
    Foxc2 Human Embryonic Kidney Week 17.1
    FoxC2 Antibody NB100-1269
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: fetal renal tissue
    Species: Human
    Verified Customer | Posted 02/10/2016
    Foxc2 staining of S-shaped body in human developing renal tissue
    FoxC2 Antibody NB100-1269

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Protocols

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FAQs

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