FOXG1 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-04084

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all FOXG1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-489 of human FOXG1 (NP_005240.3). YFTHQNQGSSSNPLIH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FOXG1 Antibody - Azide and BSA Free

Western Blot: FOXG1 AntibodyAzide and BSA Free [NBP3-04084]

Western Blot: FOXG1 AntibodyAzide and BSA Free [NBP3-04084]

Western Blot: FOXG1 Antibody [NBP3-04084] - Analysis of extracts of various cell lines, using FOXG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: FOXG1 Antibody - Azide and BSA Free [NBP3-04084]

Immunocytochemistry/ Immunofluorescence: FOXG1 Antibody - Azide and BSA Free [NBP3-04084]

Immunocytochemistry/Immunofluorescence: FOXG1 Antibody [NBP3-04084] - Analysis of U2OS cells using FOxG1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for FOXG1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500 - 1:1000

Reviewed Applications

Read 1 review rated 5 using NBP3-04084 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FOXG1

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. This gene participates in signal transduction Activin A signaling regulation, TGF-beta receptor signaling pathway, transcription factors in neurogenesis, as well as regulation of nuclear SMAD2/3 signaling. It is thought to interact with genes FOXO1, FOXO3, FOXO4, SMAD2, and SMAD3. FOXG1 is associated with diseases such as melanoma, neuronitis, glioblastoma, purpura, west syndrome, rett syndrome, medulloblastoma, hypotonia, retinitis, maternal uniparental disomy, chromosome 14, intellectual disabilities, brain malformations, multiple sclerosis, and lupus.

Alternate Names

BF-1, BF1HBF-2, BF2, BF-2, Brain factor 1, Brain factor 2, FHKL3, FKHL1forkhead-like 1, FKHL2, FKHL3, FKHL4, forkhead box G1, forkhead box G1A, forkhead box G1B, forkhead box G1C, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, forkhead-like 3, forkhead-like 4, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, FOXG1A, FOXG1Bforkhead-like 2, FOXG1C, HBF-1, hBF-2, HBF-3, HFK1HBF-G2, HFK2HBF2, HFK3KHL2, oncogene QIN, QINFKH2forkhead box protein G1

Gene Symbol

FOXG1

Additional FOXG1 Products

Product Documents for FOXG1 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FOXG1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Customer Reviews for FOXG1 Antibody - Azide and BSA Free (1)

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  • FOXG1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 11/29/2022
    Mouse forebrain lysates, band is ~52 kDa
    Block: 5% nonfat milk o/n 4 C FOXG1 antibody 1:1000 (1h RT or o/n 4 C work equally well) Secondary: 1:80,000 Sigma goat anti-rabbit (1h RT) BioRad Clarity ECL, imaged on Azure Biosystems 300 imager In some samples, an additional band shows up ~70 kDa
    FOXG1 Antibody - Azide and BSA Free NBP3-04084

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