FoxP3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-58697

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FoxP3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKW

Reactivity Notes

Mouse 87%, Rat 86%

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FoxP3 Antibody - BSA Free (NBP2-58697) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FoxP3 Antibody - BSA Free

Western Blot: FoxP3 Antibody [NBP2-58697]

Western Blot: FoxP3 Antibody [NBP2-58697]

Western Blot: FoxP3 Antibody [NBP2-58697] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody [NBP2-58697]

Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody [NBP2-58697]

Immunocytochemistry/Immunofluorescence: FoxP3 Antibody [NBP2-58697] - Staining of human cell line PC-3 shows localization to nucleoplasm. Antibody staining is shown in green.
FoxP3 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: FoxP3 Antibody - BSA Free [NBP2-58697]

Chromatin Immunoprecipitation-exo-Seq: FoxP3 Antibody - BSA Free [NBP2-58697]

ChIP-Exo-Seq composite graph for Anti-FOXP3 (NBP2-58697) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for FoxP3 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxP3

Forkhead box P3 (FoxP3), aka Scurfin and JM2, is a protein involving in immune system responses and functioning as a transcriptional regulator crucial in the development and inhibitory of regulatory T-Cells (Treg). FoxP3 is a master regulator of the Treg lineage, who requires Ahr and STAT-5 and other additional transcription factors to retains its complete function and differentiation. Scurfin remains steady and constitutively expressed in two conditions: a high level in CD25 + CD4 positive regulatory T cells and a low level in CD4 positive/CD25 negative cells. JM2 is absent in CD4 negative/CD8 positive T cells. FoxP3 defects are related to various cancers and autoimmune diseases, and X-linked syndrome (IPEX), while overexpression may also cause severe immunodeficiency.

Long Name

Forkhead Box P3

Alternate Names

AIID, DIETER, IPEX, JM2, PIDX, SCURFIN, XPID

Gene Symbol

FOXP3

Additional FoxP3 Products

Product Documents for FoxP3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FoxP3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for FoxP3 Antibody - BSA Free

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  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

  • Q: Does FoxP3 antibodies comes in lyophilized form?

    A: FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.

  • Q: What the theoretical molecular weight for FoxP3 antibodies?

    A: The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.

Showing  1 - 2 of 2 FAQs Showing All
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