FUBP1 Antibody (3H4) - Azide and BSA Free

Novus Biologicals | Catalog # H00008880-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H4

Format

Azide and BSA Free
View all FUBP1 Primary Antibodies »

Product Specifications

Immunogen

FUBP1 (NP_003893, 27 a.a. ~ 136 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPDAKKVAPQNDSFGTQLPPMHQQQSRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQI

Marker

Nucleus without Nucleoli Marker

Specificity

FUBP1 - far upstream element (FUSE) binding protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for FUBP1 Antibody (3H4) - Azide and BSA Free

Western Blot: FUBP1 Antibody (3H4) [H00008880-M01]

Western Blot: FUBP1 Antibody (3H4) [H00008880-M01]

Western Blot: FUBP1 Antibody (3H4) [H00008880-M01] - Analysis of FUBP1 expression in transfected 293T cell line by FUBP1 monoclonal antibody (M01), clone 3H4.Lane 1: FUBP1 transfected lysate(68.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody (3H4) [H00008880-M01]

Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody (3H4) [H00008880-M01]

Immunocytochemistry/Immunofluorescence: FUBP1 Antibody (3H4) [H00008880-M01] - Analysis of monoclonal antibody to FUBP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: FUBP1 Antibody (3H4) [H00008880-M01]

Immunohistochemistry-Paraffin: FUBP1 Antibody (3H4) [H00008880-M01]

Immunohistochemistry-Paraffin: FUBP1 Antibody (3H4) [H00008880-M01] - Analysis of monoclonal antibody to FUBP1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Western Blot: FUBP1 Antibody (3H4) [H00008880-M01]

Western Blot: FUBP1 Antibody (3H4) [H00008880-M01]

Western Blot: FUBP1 Antibody (3H4) [H00008880-M01] - Analysis of FUBP1 over-expressed 293 cell line, cotransfected with FUBP1 Validated Chimera RNAi ( Cat # H00008880-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FUBP1 monoclonal antibody (M01), clone 3H4 (Cat # H00008880-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: FUBP1 Antibody (3H4) [H00008880-M01]

ELISA: FUBP1 Antibody (3H4) [H00008880-M01]

ELISA: FUBP1 Antibody (3H4) [H00008880-M01] - Detection limit for recombinant GST tagged FUBP1 is approximately 0.03ng/ml as a capture antibody.

Applications for FUBP1 Antibody (3H4) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FUBP1

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

Alternate Names

far upstream element (FUSE) binding protein 1, far upstream element binding protein, far upstream element-binding protein 1, FBPDNA helicase V, FUBP, FUSE-binding protein 1, hDH V

Entrez Gene IDs

8880 (Human)

Gene Symbol

FUBP1

OMIM

603444 (Human)

UniProt

NP_003893 (Human)

Additional FUBP1 Products

Product Documents for FUBP1 Antibody (3H4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for FUBP1 Antibody (3H4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for FUBP1 Antibody (3H4) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review FUBP1 Antibody (3H4) - Azide and BSA Free and earn rewards!

Have you used FUBP1 Antibody (3H4) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies