FUBP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83918

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FUBP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPDAKKVAPQNDSFGTQLPPMHQQQRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPERSCML

Marker

Nucleus without Nucleoli Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FUBP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining in human lymph node and skeletal muscle tissues.. Corresponding FUBP1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody [NBP1-83918]

Immunocytochemistry/ Immunofluorescence: FUBP1 Antibody [NBP1-83918]

Immunocytochemistry/Immunofluorescence: FUBP1 Antibody [NBP1-83918] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.
Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918]

Immunohistochemistry-Paraffin: FUBP1 Antibody [NBP1-83918] - Staining of human endometrium shows strong nuclear positivity in glandular cells.
FUBP1 Antibody - BSA Free Western Blot: FUBP1 Antibody - BSA Free [NBP1-83918]

Western Blot: FUBP1 Antibody - BSA Free [NBP1-83918]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
FUBP1 Antibody - BSA Free Western Blot: FUBP1 Antibody - BSA Free [NBP1-83918]

Western Blot: FUBP1 Antibody - BSA Free [NBP1-83918]

Analysis in U2OS cells) transfected with control siRNA, target specific siRNA probe #1, using Anti-FUBP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
FUBP1 Antibody - BSA Free Western Blot: FUBP1 Antibody - BSA Free [NBP1-83918]

Western Blot: FUBP1 Antibody - BSA Free [NBP1-83918]

Analysis in human cell line SH-SY5Y.

Applications for FUBP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FUBP1

FUBP1 encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.

Alternate Names

far upstream element (FUSE) binding protein 1, far upstream element binding protein, far upstream element-binding protein 1, FBPDNA helicase V, FUBP, FUSE-binding protein 1, hDH V

Gene Symbol

FUBP1

Additional FUBP1 Products

Product Documents for FUBP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FUBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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