Catalog # Availability Size / Price Qty
FURA-2AM | CAS No. 108964-32-5 | Ion Indicators
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Description: Fluorescent Ca2+ indicator

Chemical Name: 2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester

Purity: ≥95%

Product Details
Citations (3)
Reviews (3)

Biological Activity

FURA-2AM is a fluorescent Ca2+ indicator. Selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. Used to determine intracellular Ca2+ concentration.

F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.

Technical Data

Soluble to 10 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for FURA-2AM

The citations listed below are publications that use Tocris products. Selected citations for FURA-2AM include:

3 Citations: Showing 1 - 3


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Reviews for FURA-2AM

Average Rating: 4.7 (Based on 3 Reviews)

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Detects store-operated Ca2+ entry after ER store depletion in RBL-2H3 cells.
By Niroj Shrestha on 11/02/2018
Application: Species: Rat

RBL-2H3 cells at a confluency of 50–60% were cultured on glass coverslip and loaded with 2 μM Fura-2AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. The Fura-2 loaded cells were excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm every 1.2 s. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.

FURA-2AM as a tool to analyse calcium transient in cardiomyocytes
By Christopher Trummer on 10/15/2018
Application: Species: Other

Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.

Detects cytosolic calcium influx changes well
By Anonymous on 11/01/2017
Application: Species: Rat

Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.

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