Chemical Name: 2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester
Biological ActivityFURA-2AM is a fluorescent Ca2+ indicator. Selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. Used to determine intracellular Ca2+ concentration.
F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
A new generation of Ca2+ indicators with greatly improved fluorescence properties.
Grynkiewicz et al.
Sildenafil inhibits human pulmonary artery smooth muscle cell proliferation by decreasing capacitative Ca2+ entry.
Wang et al.
Citations for FURA-2AM
The citations listed below are publications that use Tocris products. Selected citations for FURA-2AM include:
3 Citations: Showing 1 - 3
Niclosamide repurposed for the treatment of inflammatory airway disease.
Authors: Cabrita Et al.
JCI Insight 2019;4
KA attenuates the Na+-dependent Ca2+ overload in rabbit ventricular myocytes in vitro by inhibiting late Na+ and L-type Ca2+ currents.
Authors: Luo Et al.
Mol Neurodegener 2015;36:1327
Characterisation of AmphiAmR11, an amphioxus (Branchiostoma floridae) D2-DA-like G protein-coupled receptor.
Authors: Bayliss and Evans
PLoS One 2013;8:e80833
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Reviews for FURA-2AM
Average Rating: 4.7 (Based on 3 Reviews)
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RBL-2H3 cells at a confluency of 50–60% were cultured on glass coverslip and loaded with 2 μM Fura-2AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. The Fura-2 loaded cells were excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm every 1.2 s. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.
Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.