FURA-2AM

Tocris Bioscience | Catalog # 2220

Fluorescent Ca2+ indicator
Tocris Bioscience

Key Product Details

Description

Fluorescent Ca2+ indicator

Wavelength

Excitation = 371 nm, Emission = 474 nm
View all Ion Indicators »

Product Description

FURA-2AM is a fluorescent ratiometric Ca2+ indicator (Kd Ca2+ = 145 nM). FURA-2AM is selective for Ca2+ over other divalent cations Mg2+, Zn2+, Fe2+ and Mn2+. FURA-2AM binds to free intracellular calcium and is used to determine [Ca2+]i concentration.

This product is typically prepared in DMSO. F 127 (Cat. No. 6253) for the solubilization of FURA-2AM is also available.

Optical Data

λabs 371 nm
λem 474 nm
Extinction Coefficient (ε) 30000 M-1cm-1
Cell Permeable Yes
Application Flow Cytometry, Fluorescent Microscopy

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Product Specifications for FURA-2AM

Molecular Weight

1001.85

Formula

C44H47N3O24

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-[6-[bis[2-[(Acetyloxy)methoxy]-2-oxoethyl]amino]-5-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-benzofuranyl]-5-oxazolecarboxylic acid (acetyloxy)methyl ester

CAS Number

108964-32-5

PubChem ID

90488749

InChI Key

JTDRWQMCMPIBNJ-UHFFFAOYSA-N

SMILES

CC1=CC(OCCOC2=C(N(CC(OCOC(C)=O)=O)CC(OCOC=O)=O)C=C(OC(C4=NC=C(C(OCOC(C)=O)=O)O4)=C3)C3=C2)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Calculators

Calculate the mass, volume, or concentration required for a solution.

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Calculate the dilution required to prepare a stock solution.

x
=
x

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 9 publications citing the usage of this product.

Product Documents for FURA-2AM

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for FURA-2AM

For research use only

Customer Reviews for FURA-2AM (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used FURA-2AM?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 3 of 3 reviews Showing All
Filter By:
Clear Filters X
  • Detects store-operated Ca2+ entry after ER store depletion in RBL-2H3 cells.
    Name: Niroj Shrestha
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: RBL-2H3
    Verified Customer | Posted 11/02/2018
    RBL-2H3 cells at a confluency of 50–60% were cultured on glass coverslip and loaded with 2 μM Fura-2AM in normal tyrode solution (NT) for 40 min at room temperature in the dark. Then, the cells were washed twice and left to equilibrate for at least 20 min in NT. The Fura-2 loaded cells were excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm every 1.2 s. ER Ca2+ store was depleted with 500 nM thapsigargin which promotes store-operated Ca2+ entry into the cells upon addition of 2 mM extracellular Ca2+.
    FURA-2AM 2220
  • FURA-2AM as a tool to analyse calcium transient in cardiomyocytes
    Name: Christopher Trummer
    Species: Guinea Pig
    Assay Type: In Vitro
    Cell Line/Tissue: primary ventricular cardiomyocytes
    Verified Customer | Posted 10/15/2018
    Primary guinea pig ventricular cardiomyocytes were loaded with FURA-2AM (1 μM) and Pluronic F 127 (1%) for 30 min followed by washing with normal-Tyrode solution. Cells were stimulated at 1 Hz frequency and fluorescence intensities were measured.
    FURA-2AM 2220
  • Detects cytosolic calcium influx changes well
    Name: Anonymous
    Species: Rat
    Assay Type: In Vitro
    Cell Line/Tissue: spinal cord neurons
    Verified Customer | Posted 11/01/2017
    Used in live cell calcium imaging assay to study glutamate receptor signaling in neurons. Used at a concentration of 5 micromolar (along with pluronic). Images shows changes in fura-2 fluorescence pre (left) and post(right) glutamate stimulation.
    FURA-2AM 2220

There are no reviews that match your criteria.

Showing  1 - 3 of 3 reviews Showing All