FXYD5/Dysadherin Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82486

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all FXYD5/Dysadherin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DTTSSSSADSTIMDIQVPTRAPDAVYTELQPTSPTPTWPADETPQPQTQTQQLEGTDGPLVTDPETHKSTKAAHPTDDTTTLSERPSPSTDVQTDPQTLKPSGFHEDDPFFYDEHTLR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit FXYD5/Dysadherin Antibody - BSA Free (NBP1-82486) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for FXYD5/Dysadherin Antibody - BSA Free

Western Blot: FXYD5/Dysadherin Antibody [NBP1-82486]

Western Blot: FXYD5/Dysadherin Antibody [NBP1-82486]

Western Blot: FXYD5/Dysadherin Antibody [NBP1-82486] - Analysis in human cell lines SK-MEL-30 and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Western Blot: FXYD5/Dysadherin Antibody [NBP1-82486]

Western Blot: FXYD5/Dysadherin Antibody [NBP1-82486]

Western Blot: FXYD5/Dysadherin Antibody [NBP1-82486] - Analysis in control (vector only transfected HEK293T lysate) and FXYD5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: FXYD5/Dysadherin Antibody [NBP1-82486]

Immunocytochemistry/ Immunofluorescence: FXYD5/Dysadherin Antibody [NBP1-82486]

Immunocytochemistry/Immunofluorescence: FXYD5/Dysadherin Antibody [NBP1-82486] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: FXYD5/Dysadherin Antibody [NBP1-82486]

Immunohistochemistry-Paraffin: FXYD5/Dysadherin Antibody [NBP1-82486]

Immunohistochemistry-Paraffin: FXYD5/Dysadherin Antibody [NBP1-82486] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Applications for FXYD5/Dysadherin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FXYD5/Dysadherin

Dysadherin encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane.

Long Name

FXYD Domain-containing Ion Transport Regulator 5

Alternate Names

DYSAD, Dysadherin, EF-8, IWU1, KCT1, OIT2, PRO6241, RIC

Entrez Gene IDs

53827 (Human)

Gene Symbol

FXYD5

UniProt

Q96DB9

Additional FXYD5/Dysadherin Products

Product Documents for FXYD5/Dysadherin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for FXYD5/Dysadherin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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