G-CSFR/CD114 Antibody (PSH03-44)

Novus Biologicals | Catalog # NBP3-32378

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH03-44 expressed in HEK293
View all G-CSFR/CD114 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human G-CSFR/CD114 aa 651-836 / 836. (Uniprot: Q99062)

Localization

Cell membrane; Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for G-CSFR/CD114 Antibody (PSH03-44)

G-CSFR/CD114 Antibody (PSH03-44)

Western Blot: G-CSFR/CD114 Antibody (PSH03-44) [NBP3-32378]

Western Blot: G-CSFR/CD114 Antibody (PSH03-44) [NBP3-32378] - Western blot analysis of G-CSFR/CD114 on different lysates with Rabbit anti-G-CSFR/CD114 antibody (NBP3-32378) at 1/2,000 dilution.

Lane 1: A431 cell lysate (20 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: THP-1 cell lysate (no heat) (20 ug/Lane)
Lane 4: Jurkat cell lysate (20 ug/Lane)
Lane 5: Ramos cell lysate (20 ug/Lane)
Lane 6: Mouse placenta tissue lysate (40 ug/Lane)
Lane 7: Mouse brain tissue lysate (no heat) (40 ug/Lane)
Lane 8: Mouse testis tissue lysate (40 ug/Lane)
Lane 9: Rat placenta tissue lysate (40 ug/Lane)
Lane 10: Rat brain tissue lysate (no heat) (40 ug/Lane)
Lane 11: Rat testis tissue lysate (40 ug/Lane)
Lane 12: Rat skin tissue lysate (no heat) (40 ug/Lane)

Predicted band size: 92 kDa
Observed band size: 50-110 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for G-CSFR/CD114 Antibody (PSH03-44)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: G-CSFR/CD114

GCSF Receptor is encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, with three of the isoforms being membrane-bound and the other being secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.

Long Name

Granulocyte Colony Stimulating Factor Receptor

Alternate Names

CD114, CSF3R, Csfgr, G-CSF R, GCSFR

Gene Symbol

CSF3R

Additional G-CSFR/CD114 Products

Product Documents for G-CSFR/CD114 Antibody (PSH03-44)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for G-CSFR/CD114 Antibody (PSH03-44)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for G-CSFR/CD114 Antibody (PSH03-44)

There are currently no reviews for this product. Be the first to review G-CSFR/CD114 Antibody (PSH03-44) and earn rewards!

Have you used G-CSFR/CD114 Antibody (PSH03-44)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies