G9a/EHMT2 Antibody

Name: G9a/EHMT2 Antibody
Brand: Novus Biologicals
SKU: NBP3-18664
Price: 319 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-18664

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

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Product Specifications

Immunogen

Polyclonal antibody raised in rabbit against mouse G9a/EHMT2 (Protein G9a/EHMT2), using two KLH-conjugated synthetic peptides containing an amino acid sequence from the central part of the protein (1).

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for G9a/EHMT2 Antibody

Western Blot: G9a/EHMT2 Antibody [NBP3-18664]

Western Blot: G9a/EHMT2 Antibody [NBP3-18664] - Figure 2. Western blot analysis using the antibody directed against G9a/EHMT2 (1) Western blot was performed on whole cell lysates from mouse fibroblasts (NIH3T3) and embryonic stem cells (E14Tg2a) cells with the antibody against mouse G9a/EHMT2, diluted 1:1,000 in BSA/ PBS-Tween. The molecular weight marker (M, in kDa) is shown on the left; the location of the protein of interest (138 kDa) is indicated on the right.

ELISA: G9a/EHMT2 Antibody [NBP3-18664]

ELISA: G9a/EHMT2 Antibody [NBP3-18664] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse G9a/EHMT2. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:30,000.

Applications for G9a/EHMT2 Antibody

Application

Recommended Usage

ELISA

1:500-1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Whole Antiserum

Formulation

Whole antiserum

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: G9a/EHMT2

G9A antibody contains ankyrin repeats, suggesting involvement in intracellular protein-protein interaction. G9A is a histone methyltransferase that preferentially methylates Lys-9 of histone H3 and Lys-27 of histone H3 (in vitro). H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. G9A is probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.

Long Name

G9a Histone Methyltransferase/Euchromatic Histone-lysine N-methyltransferase 2

Alternate Names

EHMT2

Gene Symbol

EHMT2

UniProt

Q96KQ7 (Human)

Additional G9a/EHMT2 Products

Product Documents for G9a/EHMT2 Antibody

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Certificate of Analysis

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Product Specific Notices for G9a/EHMT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Notch Pathway

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