GABA-A R alpha 1 C-Terminus Antibody

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PPS022
Detection of GABAA R   alpha 1 C-Terminus by Western Blot
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Citations (1)
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GABA-A R alpha 1 C-Terminus Antibody Summary

Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Primate, Zebrafish
Specificity
Human, mouse, rat and bovine ~51 kDa GABAA R alpha 1 subunit, C-Terminus
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Fusion protein from the cytoplasmic loop GABAA R alpha 1 subunit
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of GABAA R   alpha 1 C-Terminus by Western Blot View Larger

Detection of GABAA R alpha 1 C-Terminus by Western Blot Western blot of forebrain lysates from wildtype and alpha 1 knockout animals, using approximately 5 - 7 μg of tissue per lane. Blots were incubated overnight at 2 - 8° C with anti-GABAA R, alpha 1 subunit, C-Terminus diluted 1:1000. The antibody labelled the ~51kDa alpha 1 subunit of the GABAA R (Control). The labeling was absent from a lysate prepared from alpha 1 knockout animals.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 1

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta, and gamma subunits. The rat alpha 1 isoform is a 50 - 52 kDa, 428 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is used as a regulatory mechanism. CaM kinase II is known to phosphorylate the alpha 1 subunit and regulate benzodiazepine binding. alpha 1 subunits are particularly abundant in the cerebellum and may contribute to GABA receptor distribution. In the hippocampus and amygdala, the alpha 1 subunit may contribute to amnesia.

References
  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Churn, S.B. et al. (2002) J. Neurochem. 82:1065.
  4. Kralic, J.E. et al. (2006) J. Comp. Neurol. 495:408.
  5. Sonner, J.M. et al. (2006) Mol. Pharmacol. 68:61.
Long Name
gamma-Aminobutyric Acid Type A Receptor, alpha-1 Polypeptide
Entrez Gene IDs
2554 (Human); 14394 (Mouse); 29705 (Rat); 282235 (Bovine)
Alternate Names
ECA4; EJM; EJM5GABA(A) receptor subunit alpha-1; GABA A R alpha 1; GABAAR alpha 1; GABAARa1; GABRA1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid receptor subunit alpha-1

Product Datasheets

Citation for GABA-A R alpha 1 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Grp94 Protein Delivers ?-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation
    Authors: XJ Di, YJ Wang, DY Han, YL Fu, AS Duerfeldt, BS Blagg, TW Mu
    J. Biol. Chem., 2016;291(18):9526-39.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: WB

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