GABA-A R alpha 2 Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of GABAAR alpha 2 by Western Blot. Western blot of 10 μg of rat cortex lysates showing immunolabeling of the ~53 kDa alpha 2 subunit of the GABAAR.
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Background: GABA-A R alpha 2
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha 2 isoform is a 53 kDa, 423 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is likely to be used as a regulatory mechanism. The alpha 2 subunit is known to be phosphorylated on Ser/Thr residues. Rat alpha 2 has multiple isoforms, all involving the 5′ UTR. These may dictate alpha 2 levels within the cell. This receptor subunit is particularly abundant in forebrain areas and is a potential target of anabolic steroids. Behavioral alterations associated with steroid abuse may be a manifestation of alpha 2 beta 3 gamma 2L receptor activation.
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- Yang, P. et al. (2005) Neuropharmacology 49:300.
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- Bureau, M.H. and J.J. Laschet (1995) J.Biol. Chem. 270:26482.
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