GABA-A R alpha 2 Antibody

  • Species Reactivity
    Human, Mouse, Rat, Bovine, Canine, Primate
  • Specificity
    Human, mouse, rat and bovine ~53 kDa GABAA R alpha 2 subunit
  • Source
    Polyclonal Rabbit Serum
  • Purification
    Antigen Affinity-purified
  • Immunogen
    GABAA R alpha 2 subunit specific peptide
  • Formulation
    50 μL unpurified neat rabbit serum.
  • Label
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GABAA R alpha 2 by Western Blot. Western blot of 10 μg of rat cortex lysates showing immunolabeling of the ~53 kDa alpha 2 subunit of the GABAA R.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R alpha 2
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha 2 isoform is a 53 kDa, 423 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is likely to be used as a regulatory mechanism. The alpha 2 subunit is known to be phosphorylated on Ser/Thr residues. Rat alpha 2 has multiple isoforms, all involving the 5′ UTR. These may dictate alpha 2 levels within the cell. This receptor subunit is particularly abundant in forebrain areas and is a potential target of anabolic steroids. Behavioral alterations associated with steroid abuse may be a manifestation of alpha 2 beta 3 gamma 2L receptor activation.
  • References:
    1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
    2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
    3. Khrestchatisky, M. et al. (1991) J. Neurochem. 56:1717.
    4. Yang, P. et al. (2005) Neuropharmacology 49:300.
    5. Tian, H. et al. (2005) Mol. Brain Res. 137:174.
    6. Bureau, M.H. and J.J. Laschet (1995) J.Biol. Chem. 270:26482.
  • Long Name:
    gamma-Aminobutyric Acid Type A Receptor, alpha-2 Polypeptide
  • Entrez Gene IDs:
    2555 (Human); 14395 (Mouse); 289606 (Rat); 282236 (Bovine)
  • Alternate Names:
    GABA(A) receptor subunit alpha-2; GABAAR alpha 2; GABAARa2; GABRA2; gamma-aminobutyric acid (GABA) A receptor, alpha 2; gamma-aminobutyric acid receptor subunit alpha-2
Related Research Areas
Molecular Weight Markers
Description Application Cat# Citations Images  

Full-Range Molecular Weight Marker, Biotinylated

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