GABAA R  gamma 2 C-Terminus Antibody

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PPS029
GABAA R  gamma 2 C-Terminus Antibody in Data
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GABAA R  gamma 2 C-Terminus Antibody Summary

Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate
Specificity
Human, mouse, rat, and bovine ~44 - 47 kDa GABAA R gamma 2 subunit, C-Terminus
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Fusion protein from the cytosolic loop of the rat GABAA R gamma 2 subunit, C-Terminus
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of GABA<sub>A</sub>R  gamma 2 C-Terminus Western Blot. View Larger

Detection of GABAAR gamma 2 C-Terminus Western Blot. Western blot of rat hippocampal lysate. The blot was incubated overnight at 2 - 8° C with anti-GABAAR gamma 2 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~44 - 47 kDa gamma 2 subunit of the GABAAR.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R gamma 2

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat gamma 2 isoform is a 48 kDa, 436 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 30 - 233). The gamma 2 subunit is part of the most common GABAA receptor combination in the mammalian brain ( alpha 1 beta 2 gamma 2). GABA binds at alpha -beta interfaces, while benzodiazepine binds to alpha -gamma interfaces. There are two splice forms, the longest contains a consensus phosphorylation site in the second cytoplasmic domain, and a short form that shows an absence of this site through a deletion of aa 376 - 383. PKC phosphorylates the long form at S381, while both the short and long forms are phosphorylated at S365. Phosphorylation blocks receptor activity. The gamma 2 subunits are also palmitoylated at multiple sites on cysteines that lay between aa 415 - 461, facilitating membrane trafficking.

References
  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
  4. Krishek, B.J. et al. (1994) Neuron 12:1081.
  5. Keller, C.A. et al. (2004) J. Neurosci. 24:5881.
  6. Moss, S.J. et al. (1992) J. Biol. Chem. 267:14470.
Long Name
gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide
Entrez Gene IDs
2566 (Human); 14406 (Mouse); 29709 (Rat); 282240 (Bovine)
Alternate Names
CAE2; ECA2; GABA A R gamma 2; GABA(A) receptor subunit gamma-2; GABAAR gamma 2; GABAARg2; GABRG2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; gamma-aminobutyric acid receptor subunit gamma-2; GEFSP3

Product Datasheets

Citation for GABAA R  gamma 2 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

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