Detection of GABAA R gamma 2 C-Terminus Western Blot.
Western blot of rat hippocampal lysate. The blot was incubated overnight at 2 - 8° C with anti-GABAA R gamma 2 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~44 - 47 kDa gamma 2 subunit of the GABAA R.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R gamma 2
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat gamma 2 isoform is a 48 kDa, 436 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 30 - 233). The gamma 2 subunit is part of the most common GABAA receptor combination in the mammalian brain ( alpha 1 beta 2 gamma 2). GABA binds at alpha -beta interfaces, while benzodiazepine binds to alpha -gamma interfaces. There are two splice forms, the longest contains a consensus phosphorylation site in the second cytoplasmic domain, and a short form that shows an absence of this site through a deletion of aa 376 - 383. PKC phosphorylates the long form at S381, while both the short and long forms are phosphorylated at S365. Phosphorylation blocks receptor activity. The gamma 2 subunits are also palmitoylated at multiple sites on cysteines that lay between aa 415 - 461, facilitating membrane trafficking.
Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
Krishek, B.J. et al. (1994) Neuron 12:1081.
Keller, C.A. et al. (2004) J. Neurosci. 24:5881.
Moss, S.J. et al. (1992) J. Biol. Chem. 267:14470.
gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide
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