GAD2/GAD65 Antibody (GAD2/8394R)

Novus Biologicals | Catalog # NBP3-20343

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # GAD2/8394R
View all GAD2/GAD65 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human GAD2/GAD65 protein fragment (around aa 1-200) (exact sequence is proprietary)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human pancreas or brain (IHC).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for GAD2/GAD65 Antibody (GAD2/8394R)

GAD2/GAD65 Antibody (GAD2/8394R)

GAD2/GAD65 Antibody (GAD2/8394R)

Formalin-fixed, paraffin-embedded human pancreas stained with GAD2/GAD65 antibody (GAD2/8394R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
GAD2/GAD65 Antibody (GAD2/8394R)

GAD2/GAD65 Antibody (GAD2/8394R)

Formalin-fixed, paraffin-embedded human pancreas stained with GAD2/GAD65 antibody (GAD2/8394R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for GAD2/GAD65 Antibody (GAD2/8394R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GAD2/GAD65

Antibodies to glutamic acid decarboxylase-65 (GAD65) are present in autoimmune disorders such as insulin-dependent (type 1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.

Long Name

Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa)

Alternate Names

GAD65

Gene Symbol

GAD2

Additional GAD2/GAD65 Products

Product Documents for GAD2/GAD65 Antibody (GAD2/8394R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GAD2/GAD65 Antibody (GAD2/8394R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GAD2/GAD65 Antibody (GAD2/8394R)

There are currently no reviews for this product. Be the first to review GAD2/GAD65 Antibody (GAD2/8394R) and earn rewards!

Have you used GAD2/GAD65 Antibody (GAD2/8394R)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies