GAK Antibody (4C10) - Azide and BSA Free

Novus Biologicals | Catalog # H00002580-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C10

Format

Azide and BSA Free
View all GAK Primary Antibodies »

Product Specifications

Immunogen

GAK (AAH08668, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SAVAPTPATEGPLFSPGGQPAPCGSQASWTKSQNPDPFADLGDLSSGLQGSPAGFPPGGFIPKTATTPKGSSSWQTSRPPAQGASWPPQAKPPPKACTQP

Localization

Cytoplasm, perinuclear region. Golgi apparatus, trans Golgi network. Cell junction, focal adhesion. Localizes to the perinuclear area and to the trans Golgi network. Also seen on the plasma membrane, probably at focal adhesions.

Specificity

GAK - cyclin G associated kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for GAK Antibody (4C10) - Azide and BSA Free

Western Blot: GAK Antibody (4C10) [H00002580-M01]

Western Blot: GAK Antibody (4C10) [H00002580-M01]

Western Blot: GAK Antibody (4C10) [H00002580-M01] - Analysis of GAK expression in transfected 293T cell line by GAK monoclonal antibody (M01), clone 4C10.Lane 1: GAK transfected lysate (Predicted MW: 44 KDa).Lane 2: Non-transfected lysate.
Western Blot: GAK Antibody (4C10) [H00002580-M01]

Western Blot: GAK Antibody (4C10) [H00002580-M01]

Western Blot: GAK Antibody (4C10) [H00002580-M01] - GAK monoclonal antibody (M01), clone 4C10 Analysis of GAK expression in Hela S3 NE.
Immunoprecipitation: GAK Antibody (4C10) [H00002580-M01]

Immunoprecipitation: GAK Antibody (4C10) [H00002580-M01]

Immunoprecipitation: GAK Antibody (4C10) [H00002580-M01] - Analysis of GAK transfected lysate using anti-GAK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GAK monoclonal antibody.
Sandwich ELISA: GAK Antibody (4C10) [H00002580-M01] - Detection limit for recombinant GST tagged GAK is approximately 0.03ng/ml as a capture antibody.

Applications for GAK Antibody (4C10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GAK

In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5.

Long Name

Cyclin G-associated Kinase

Alternate Names

Auxillin 2

Entrez Gene IDs

2580 (Human)

Gene Symbol

GAK

OMIM

602052 (Human)

UniProt

AAH08668 (Human)

Additional GAK Products

Product Documents for GAK Antibody (4C10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for GAK Antibody (4C10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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