Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-09032

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RG9-35

Format

Azide and BSA Free
View all Galectin-9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising rats with recombinant mouse galectin-9.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free

Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free

Flow Cytometry: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] -

Flow Cytometry: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] - NIH3T3 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-09032 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] -

Immunocytochemistry /Immunofluorescence: Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free [NBP3-09032] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with NBP3-09032 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-09032, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Galectin-9

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin 9 is an S-type lectin and an eosinophil chemoattractant (ECA) produced by antigen-activated T cells. Galectin 9 is involved in apoptosis, is thought to be a prognostic factor for breast cancer and is also overexpressed in Hodgkin's disease tissue. The protein has N- and C- terminal carbohydrate-binding domains connected by a link peptide. Multiple alternatively spliced transcript variants have been found for this gene.

Alternate Names

Ecalectin, GAL9, Galectin9, LGALS9

Gene Symbol

LGALS9

Additional Galectin-9 Products

Product Documents for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Galectin-9 Antibody (RG9-35) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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