GALT Antibody (PSH02-88)

Novus Biologicals | Catalog # NBP3-32364

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH02-88 expressed in HEK293
View all GALT Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within human GALT aa 1-379 / 379. (Uniprot: P07902)

Localization

Cytosol, Golgi apparatus, cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GALT Antibody (PSH02-88)

GALT Antibody (PSH02-88)

Western Blot: GALT Antibody (PSH02-88) [NBP3-32364]

Western Blot: GALT Antibody (PSH02-88) [NBP3-32364] - Western blot analysis of GALT on different lysates with Rabbit anti-GALT antibody (NBP3-32364) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: A549 cell lysate (15 µg/Lane)
Lane 4: K-562 cell lysate (15 µg/Lane)
Lane 5: THP-1 cell lysate (15 µg/Lane)

Predicted band size: 43 kDa
Observed band size: 43 kDa

Exposure time:
Lane 1-5 (left): 1 minute 34 seconds;
Lane 1-5 (right): 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for GALT Antibody (PSH02-88)

Application
Recommended Usage

Western Blot

1:2000-1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GALT

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Long Name

galactose-1-phosphate uridylyltransferase

Alternate Names

EC 2.7.7.12, Gal-1-P uridylyltransferase, galactose-1-phosphate uridyl transferase, galactose-1-phosphate uridylyltransferase, UDP-glucose--hexose-1-phosphate uridylyltransferase

Gene Symbol

GALT

Additional GALT Products

Product Documents for GALT Antibody (PSH02-88)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GALT Antibody (PSH02-88)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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