GAPDH Antibody

Novus Biologicals | Catalog # NB300-322

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Primate

Cited:

Human, Mouse, Rat, Avian - Chicken, Primate

Predicted:

Amphibian (100%), Avian (100%), Bovine (100%), Canine (100%), Chinese Hamster (100%), Ferret (100%), Fish (100%), Golden Syrian Hamster (100%), Guinea Pig (100%), Hamster (100%), Porcine (100%), Rabbit (100%), Sheep (100%), Squirrel (100%), Turkey (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GAPDH Primary Antibodies »

Product Specifications

Immunogen

This GAPDH antibody was developed against an epitope between residues 150 and 200 of human GAPDH using the numbering given in entry NP_002037.2 (GeneID 2597).

Reactivity Notes

Chicken reactivity reported in scientific literature (Youngworth IA et al).

Marker

Cytosolic Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit GAPDH Antibody (NB300-322) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, Simple Western and IP. Anti-GAPDH Antibody: Cited in 42 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for GAPDH Antibody

Simple Western: GAPDH Antibody [NB300-322]

Simple Western: GAPDH Antibody [NB300-322]

Simple Western: GAPDH Antibody [NB300-322] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.
Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-322 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322] - Detection of Human and Mouse GAPDH (theoretical molecular weight: 36 kDa) by Western Blot. Samples: Whole cell lysate from mouse NIH3T3 (5, 15 and 50 ug) and human HeLa (H; 50 ug) cells. Antibody: Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: GAPDH Antibody [NB300-322]

Immunohistochemistry: GAPDH Antibody [NB300-322]

Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB
Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322]

GAPDH-Antibody-Western-Blot-NB300-322-img0011.jpg
Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-322]

Immunocytochemistry/ Immunofluorescence: GAPDH Antibody [NB300-322]

Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-322] - GAPDH detection in HeLa cells with ICC-IF application using NB300-322, visualized with DyLight Fluor 488.
Immunohistochemistry: GAPDH Antibody [NB300-322]

Immunohistochemistry: GAPDH Antibody [NB300-322]

Immunohistochemistry: GAPDH Antibody [NB300-322] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-322). Detection: DAB
Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322] - Mouse DRG stained at 1:2000 dilution. Image provided by verified customer review.
Western Blot: GAPDH Antibody [NB300-322]

Western Blot: GAPDH Antibody [NB300-322]

GAPDH-Antibody-Western-Blot-NB300-322-img0010.jpg
Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322]

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322]

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of human lung carcinoma using NB300-322 at a dilution of 1:200.
Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322]

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322]

Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-322] - IHC-P detection of GAPDH in formalin fixed paraffin embedded section of mouse squamous cell carcinoma using NB300-322 at a dilution of 1:200.
GAPDH Antibody

Simple Western: GAPDH Antibody [NB300-322] -

Time-dependent effects of subacute administration of effective dose of escitalopram (5 mg/kg/day) for 3 & 7 days on expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. ** p < 0.01 vs. control by one-way analysis of variance with Tukey’s post-hoc test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Concentration-dependent effects of subchronic administration of ZTP on Cx43 protein expression in the cytosol fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Concentration-dependent effects of ZTP (100, 300 & 1000 nM) on Cx43 expression in the cytosol fraction of the primary cultured astrocytes were analysed by one-way ANOVA with Tukey’s (wholly significant difference) post hoc test (* p < 0.05 vs. ZTP free (0)). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Effects of acute (120 min) administration of supratherapeutic concentration of ZTP (1000 nM) & chronic (14 days) administration of therapeutically relevant concentrations of ZTP (300 nM) on Cx43 protein expression in the plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by student T-test (* p < 0.05 vs. control: ZTP free). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Interaction between subchronic administration of supratherapeutic concentration of ZTP & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction (A) & their pseudogel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of ZTP (1000 nM) & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analysed by two-way ANOVA with Tukey’s (wholly significant difference) post hoc test (** p < 0.01 vs. control, @@ p < 0.01 vs. non). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34832898), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Simple Western: GAPDH Antibody [NB300-322] -

Simple Western: GAPDH Antibody [NB300-322] - Effects of subchronic nicotine administration on the expression of phosphorylated protein kinase B (pAkt) & phosphorylated extracellular signal-regulated kinase (pErk) in the plasma membrane fraction of OFC. Effects of the systemic subchronic administration of nicotine (50 mg/kg/day for seven days) on pAkt & pErk expression in the OFC plasma membrane fraction before four week of age (A,C) & after 12 week of age (B,D), ADSHE onset of the wild-type & S286L-TG & pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of pErk & pAkt. * p < 0.05, ** p < 0.01 vs. wild-type, & @ p < 0.05, @@ p < 0.01 vs. nicotine-free (non) by two-way ANOVA with Tukey’s multiple comparison. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33143372), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Simple Western: GAPDH Antibody [NB300-322] -

Simple Western: GAPDH Antibody [NB300-322] - Effects of the subacute administration of effective doses of vortioxetine (2.5 mg/kg/day), escitalopram (5 mg/kg/day), & lurasidone (3 mg/kg/day) for 3 days on the expression of 5-HT7R in the thalamic plasma membrane fraction (Panel (A)). Ordinate: mean ± SD (n = 6) of the relative protein level of 5-HT7R in the thalamic plasma membrane fraction. Panel (B) indicates the pseudo-gel images using capillary immunoblotting. * p < 0.05, ** p < 0.01 vs. the control by Student’s t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33572981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase cell motility.(A): HeLa cells were transduced with Lenti-control or Lenti-Tax viral particles. When cells reached 100% confluence, a wound was made in the cell monolayer & pictures were taken at 0 h & up to 12 h post-wounding. Measurements between the 2 fronts of migration were performed using image-J software (NIH, USA). (B): Percentage of healing during a 12 h kinetic. (C): Western blot analyses were performed on 70 µg of cellular extracts transduced by Lenti-control or Lenti-Tax viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem expression is sufficient to increase chemokinesis & chemotaxis.(A, C): MOLT4 cells were transduced with Lenti-control or Lenti-GEM. Forty-eight hours later, 5.105 cells were collected & loaded on a 5 µm permeable Transwell filter in absence or presence of SDF1/CXCL12 (150 ng/ml). Twenty-four hours later, cell migration was quantified by flow cytometry (flow cytometer Facscalibur4c+HTS (BD biosciences)). (B, D): Western blot analyses were performed on 70 µg of cellular extracts from MOLT4 cells transduced by Lenti-control or Lenti-GEM viral particles. Membranes were probed with anti-Gem (1∶2,000) or anti-GAPDH (1∶1,000) antibody. ***: significantly different, p<0.0001, Student's t-test. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Effects of subchronic administration of therapeutic-relevant concentration of antipsychotics, CLZ (3 μM), QTP (1 μM) & BPZ (0.3 μM), on Cx43 protein expression in the plasma fraction (A) & their pseudo-gel images, using capillary immunoblotting (B). Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Interaction between acute administration of therapeutic-relevant concentration of antipsychotics & Akt inhibitor (DEBC) on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (500 μM) (A). Interaction between subchronic administration of therapeutic-relevant concentration of antipsychotics & DEBC on Cx43 protein expression in the astroglial plasma membrane fraction, after subchronic administration of therapeutic-relevant concentration of VPA (B). Lower panels indicate their pseudo-gel images, using capillary immunoblotting. Ordinate: mean ± SD (n = 6) of the relative protein level of Cx43 (per GAPDH). Effects of antipsychotics & Akt inhibitor (DEBC: 10 μM) on Cx43 expression in the plasma membrane fraction of the primary cultured astrocytes were analyzed by MANOVA with Tukey’s post hoc test (** p < 0.01 vs. non, @@ p < 0.01 vs. control). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/34070699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Western Blot: GAPDH Antibody [NB300-322] -

Western Blot: GAPDH Antibody [NB300-322] - Gem is overexpressed in T- & non-T-Tax-expressing cells as well as in HTLV-1 infected cells.(A): Total RNA was extracted from 293T cells transduced with Lenti-control (lane 2) or Lenti-Tax (lane 3) & RT-PCR was performed using gem or GADPH specific primers. Lane 1 is a control of extraction. (B, C): Western blot analyses were performed with 70 µg of proteins from (B) 293T or (C) MOLT4 cells transduced by Lenti-control or Lenti-Tax vectors (72 h post-transduction). (D): JPX-9 cells were grown for 24 h or 48 h in the presence of ZnCl2 (120 µM). Western blot analyses were performed with 70 µg of JPX-9 cellular extracts. (E): Western blot analyses were performed with 70 µg of cellular extracts obtained from non-infected (CEM, Jurkat & MOLT4) or HTLV-1-infected (C8166, C91/PL, Hut102 & MT2) T-lymphocytes. (B, C, D, E): Membranes were probed with anti-Gem (1∶2,000) & anti-GAPDH (1∶1,000) or anti-Tax Tab 172 (1∶500) antibodies. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1003917), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
GAPDH Antibody

Simple Western: GAPDH Antibody [NB300-322] -

Simple Western: GAPDH Antibody [NB300-322] - Age-dependent fluctuations of pannexin1 expression in the plasma membrane in OFC of wild type and S286L-TG. Panel (A) indicates expressions of pannexin1 in the plasma mem-brane fraction of OFC of wild type (brown columns) and S286L-TG (green columns) at 4, 7, and 10 weeks of age, respectively. Ordinates indicate the mean ± SD (n = 6) of relative expression of pannexin1 per GAPDH, and abscissas indicate ages (weeks). ** p < 0.01, relative to pannexin1 expression at 4 weeks of age, @@ p < 0.01, relative to the wild type of the same age using two-way ANOVA with Scheffe’s post hoc test. F value was [Fage(2,30) = 0.7 (p > 0.1), Fgenotype(1,30) = 30.7 (p < 0.01), Fage*genotype(2,30) = 11.0 (p < 0.01)]. Panel (B) indicates the pseudo-gel images of pannexin1 and GAPDH using capillary immunoblotting. Image collected and cropped by CiteAb under a CC-BY license from the following publication: Age-Dependent Activation of Pannexin1 Function Contributes to the Development of Epileptogenesis in Autosomal Dominant Sleep-related Hypermotor Epilepsy Model Rats. Int J Mol Sci (2024). Not internally tested by Novus Biologicals.
GAPDH Antibody

Simple Western: GAPDH Antibody [NB300-322] -

Simple Western: GAPDH Antibody [NB300-322] - Astroglial expression in the plasma membrane evoked by ripple burst and fast ripple burst stimulations in wild type and S286L-TG. Panel (A) indicates expression of pannexin1 in the astroglial plasma membrane fraction. Ordinates indicate the mean ± SD (n = 6) of relative expression of pannexin1 per GAPDH. Gray and green bars indicate pannexin1 expression in astrocytes of wild type and S286L-TG after chronic fast ripple-evoked stimulation, respectively. * p < 0.05, ** p < 0.01 using two-way ANOVA with Scheffe’s post hoc test. F value was [Fgenotype(1,20) = 16.6 (p < 0.01), Fage(1,20) = 2.0 (p > 0.1), Fgenotype*age(1,20) = 5.4 (p < 0.05)]. Panel (B) indicates the pseudo-gel images of P2X7R and GAPDH, using capillary immunoblotting. Image collected and cropped by CiteAb under a CC-BY license from the following publication: Age-Dependent Activation of Pannexin1 Function Contributes to the Development of Epileptogenesis in Autosomal Dominant Sleep-related Hypermotor Epilepsy Model Rats. Int J Mol Sci (2024). Not internally tested by Novus Biologicals.

Applications for GAPDH Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:100-1:500

Simple Western

1:100

Western Blot

1:2000-1:10000
Application Notes

This GAPDH antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry-Paraffin applications. For IHC, antigen retrieval with citrate buffer pH6.0 is recommended for formalin fixed paraffin embedded tissue sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Brain, separated by Size, antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-322 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GAPDH

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968

Long Name

Glyceraldehyde-3-phosphate Dehydrogenase

Alternate Names

G3PDH

Entrez Gene IDs

2597 (Human); 14433 (Mouse)

Gene Symbol

GAPDH

UniProt

P04406 (Human)

Additional GAPDH Products

Product Documents for GAPDH Antibody

Certificate of Analysis

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Product Specific Notices for GAPDH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for GAPDH Antibody (2)

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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse DRG
    Species: Mouse
    Verified Customer | Posted 02/28/2014
    GAPDH in mouse DRG
    GAPDH Antibody NB300-322
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: PC3 Whole Cell Lysate (Human prostate cancer cell)
    Species: Human
    Verified Customer | Posted 09/29/2011

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GAPDH Antibody

Showing  1 - 5 of 9 FAQs Showing All

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

  • Q: Do you offer a smaller size for the GAPDH antibodies?

    A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

  • Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?

    A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.

  • Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.

    A: Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.

  • Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

    A: For homogenized tissue, beta-actin and GAPDH are both fine.

  • Q: I would like to ensure NB300-322 can be used with samples from grouper.

    A: Our GAPDH antibody with catalogue number NB300-322 has been epitope mapped and found to recognise a region between residues 150 and 200 of human GAPDH. Unfortunately we have not yet tested this antibody specifically against samples from Grouper.

  • Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?

    A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.

  • Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?

    A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.

  • Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.

    A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul. 

  • Q: What secondary antibody should I use for visualization of the GAPDH antibody?

    A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.

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