Recombinant Human GAPDH GST (N-Term) Protein
Novus Biologicals | Catalog # H00002597-P02
![Western Blot: Recombinant Human GAPDH GST (N-Term) Protein [H00002597-P02]](https://resources.rndsystems.com/images/products/Recombinant-Human-GAPDH-Protein-Western-Blot-H00002597-P02-img0003.jpg "Western Blot: Recombinant Human GAPDH GST (N-Term) Protein [H00002597-P02]")
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Key Product Details
Source
Wheat germ
Tag
GST (N-Term)
Applications
Western Blot, ELISA, Affinity Purification, Microarray, SDS-PAGE
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Product Specifications
Description
A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-335 of Human GAPDH
Source: Wheat Germ (in vitro)
Amino Acid Sequence: MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Purity
>80% by SDS-PAGE and Coomassie blue staining
Predicted Molecular Mass
62.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Protein / Peptide Type
Recombinant Protein
Reviewed Applications
Read 1 review rated 4 using H00002597-P02 in the following applications:
Scientific Data Images for Recombinant Human GAPDH GST (N-Term) Protein
Western Blot: Recombinant Human GAPDH GST (N-Term) Protein [H00002597-P02]
Western Blot: Recombinant Human GAPDH Protein [H00002597-P02] - Analysis of GAPDH in GAPDH recombinant protein. Image from verified customer review.![SDS-PAGE: Recombinant Human GAPDH GST (N-Term) Protein [H00002597-P02]](https://resources.rndsystems.com/images/products/Recombinant-Human-GAPDH-Protein-SDS-Page-H00002597-P02-img0004.jpg "SDS-PAGE: Recombinant Human GAPDH GST (N-Term) Protein [H00002597-P02]")
SDS-PAGE: Recombinant Human GAPDH GST (N-Term) Protein [H00002597-P02]
SDS-Page: Recombinant Human GAPDH Protein [H00002597-P02] - 12.5% SDS-PAGE Stained with Coomassie Blue. Theoretical molecular weight: 62.5 kDa.Formulation, Preparation, and Storage
H00002597-P02
| Preparation Method | in vitro wheat germ expression system |
| Formulation | 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer. |
| Preservative | No Preservative |
| Concentration | Please see the vial label for concentration. If unlisted please contact technical services. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -80C. Avoid freeze-thaw cycles. |
Background: GAPDH
References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908
2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079
3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169
4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139
5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968
Long Name
Glyceraldehyde-3-phosphate Dehydrogenase
Alternate Names
G3PDH
Gene Symbol
GAPDH
Additional GAPDH Products
Product Documents for Recombinant Human GAPDH GST (N-Term) Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Recombinant Human GAPDH GST (N-Term) Protein
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Recombinant Human GAPDH GST (N-Term) Protein
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FAQs for Recombinant Human GAPDH GST (N-Term) Protein
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Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
-
Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
-
Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
-
Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
-
Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
-
Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
-
Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
-
Q: Do you offer a smaller size for the GAPDH antibodies?
A: We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.
-
Q: I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
A: This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
-
Q: I want to know why GAPDH is used as a loading control antibody in WB techniques.
A: Our GAPDH antibody makes a good loading control because GAPDH is a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
-
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.
A: For homogenized tissue, beta-actin and GAPDH are both fine.
-
Q: I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody we already had but it didn't work. What can you recommend?
A: Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
-
Q: We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
A: GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are interested in. GAPDH antibody is a good choice for low to mid MW targets, as GAPDH detects around 35 kDa.
-
Q: We received GAPDH antibody NB600-502 and there was only about 15µl of antibody in the tube. I was under the impression this should have been 100µl.
A: The unit size of this product is 0.1 mg with a concentration of 6.7 mg/ml. Hence the volume should be no less than 14.9 ul.
-
Q: What secondary antibody should I use for visualization of the GAPDH antibody?
A: This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
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