Gasdermin D Antibody (5U7R7)

Novus Biologicals | Catalog # NBP3-16070

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5U7R7 expressed in HEK293
View all Gasdermin D Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human Gasdermin D (NP_079012.3). DGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gasdermin D Antibody (5U7R7)

Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]

Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]

Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of A-431 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Western Blot: Rabbit GSDMDC1 mAb (5U7R7) [NBP3-16070] -

Western Blot: Rabbit GSDMDC1 mAb (5U7R7) [NBP3-16070] - Analysis of extracts of THP-1, using GSDMD antibody at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.
GSDMDC1 Antibody (5U7R7)

Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070] -

Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070] - Western blot analysis of lysates from PC-3 cells using GSDMDC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.

Applications for Gasdermin D Antibody (5U7R7)

Application
Recommended Usage

Western Blot

1:500 -1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gasdermin D

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Alternate Names

DF5L, DFNA5L, gasdermin domain containing 1, Gasdermin domain-containing protein 1, gasdermin-D, GSDMDC1

Gene Symbol

GSDMD

Additional Gasdermin D Products

Product Documents for Gasdermin D Antibody (5U7R7)

Certificate of Analysis

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Product Specific Notices for Gasdermin D Antibody (5U7R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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