Gasdermin D Antibody (8K8C6)

Novus Biologicals | Catalog # NBP3-33450

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 8K8C6
View all Gasdermin D Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 276-484 of human Gasdermin D)

Sequence:
GVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Gasdermin D Antibody (8K8C6)

GSDMDC1 Antibody (8K8C6)

Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] -

Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
GSDMDC1 Antibody (8K8C6)

Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] -

Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 10 ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Applications for Gasdermin D Antibody (8K8C6)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:1000 - 1:3000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Gasdermin D

Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Alternate Names

DF5L, DFNA5L, gasdermin domain containing 1, Gasdermin domain-containing protein 1, gasdermin-D, GSDMDC1

Gene Symbol

GSDMD

Additional Gasdermin D Products

Product Documents for Gasdermin D Antibody (8K8C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Gasdermin D Antibody (8K8C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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