GAT3 Antibody - BSA Free

Western Blot: GAT3 Antibody - BSA Free [NBP1-91920] -

Glutamate and GABA transporter levels in the synaptosomal fraction. (A) Representative western blots of glutamate and GABA transporter protein expression in wild-type, wild-type/dnSNARE, BACHD and BACHD/dnSNARE mice. (B) Western blot quantification of the transporters (shown are n=3 independent samples). HTT was probed to confirm the genotype of sample mice on the same blot probed for GAT1 (same beta -actin loading control as shown for GAT1). No significant difference was observed in GLT1, GAT3 and GAT1 (n=5-6/genotype). Data are mean+/-s.e.m. Differences among the groups were assessed by one-way ANOVA followed by Tukey's HSD multiple comparison procedure. Non-significant P-values are not displayed on graphs. Refer to Table 1 for all P-values. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39526491), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GAT3 Antibody - BSA Free [NBP1-91920] -

Expression of neurotransmitter transporters. (A,B) Representative RNAscope images of Slc6a1, Slc6a11and Gja1 in the striatum. Scale bars: 50 μm. The numbers of Gja1-positive cells co-labeled with Slc6a1 (encoding GAT1) or Slc6a11 (encoding GAT3) were counted. (C) Representative western blots of GLT1, GAT3 and GAT1 protein expression in the striatum of wild-type, wild-type/dnSNARE, BACHD and BACHD/dnSNARE mice (each lane represents a single sample; each sample is pooled from three mice of the same genotype). (D) Western blot quantification of GLT1, GAT3 and GAT1 (n=6/genotype). WT, wild type. The loading control beta -actin shown for GAT1 in C is the same as the beta -actin shown for Cx43 in Fig. 5A. The same membrane was probed for beta -actin, GAT1 and Cx43. The loading control beta -actin shown for GLT1 in C is the same as the beta -actin used for GFAP in Fig. 5A. GLT1 and GFAP were probed on the same blot membrane. Data are mean+/-s.e.m. Differences among the groups were assessed by one-way ANOVA followed by Tukey's HSD multiple comparison procedure. Non-significant P-values are not displayed on graphs. Refer to Table 1 for all P-values. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39526491), licensed under a CC-BY license. Not internally tested by Novus Biologicals.