Rat GCLC ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-53086

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.33 ng/mL (example only; lot dependent)

Assay Range

0.781 - 50 ng/mL (example only; lot dependent)
View all GCLC ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Rat GCLC ELISA Kit (Colorimetric)

Rat GCLC ELISA Kit (Colorimetric) ELISA: Rat GCLC ELISA Kit (Colorimetric) [NBP3-53086]

ELISA: Rat GCLC ELISA Kit (Colorimetric) [NBP3-53086]

Kit Contents for Rat GCLC ELISA Kit (Colorimetric)

  • Pre-coated 96T strip plate, Plate sealer for 96 wells, Standard, Diluent Buffer, Detection Reagent A, Detection Reagent B, TMB Substrate, Stop Solution, Wash Buffer (30 x concentrate), Instruction manual

Preparation and Storage

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GCLC

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Long Name

Glutamate--cysteine ligase catalytic subunit

Alternate Names

EC 6.3.2.2, Gamma-ECS, GCS heavy chain, GLCL, GLCLC

Gene Symbol

GCLC

Additional GCLC Products

Product Documents for Rat GCLC ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Rat GCLC ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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